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Identification of a Cucumber Mosaic Virus from Cucurbita pepo on New Reclamation Land in Egypt and the Changes Induced in Pumpkin Plants

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  • Wael Fathy Shehata

    (Department of Agricultural Biotechnology, College of Agriculture and Food Sciences, King Faisal University, Hofuf 31982, Saudi Arabia
    Plant Production Department, College of Environmental Agricultural Science, Arish University, Arish 45511, Egypt)

  • Zafar Iqbal

    (Central Laboratories, Hofuf 31982, Saudi Arabia)

  • Tarek Elsayed Abdelbaset

    (Virus and Phytoplasma Research Department, Plant Pathology Research Institute, Agricultural Research Center, Giza 12619, Egypt)

  • Khalied Ibrahiem Saker

    (Virus and Phytoplasma Research Department, Plant Pathology Research Institute, Agricultural Research Center, Giza 12619, Egypt)

  • Ahmed Elnabawy El Shorbagy

    (Virus and Phytoplasma Research Department, Plant Pathology Research Institute, Agricultural Research Center, Giza 12619, Egypt)

  • Ahmed Mohamed Soliman

    (Virus and Phytoplasma Research Department, Plant Pathology Research Institute, Agricultural Research Center, Giza 12619, Egypt)

  • Muhammad Naeem Sattar

    (Central Laboratories, Hofuf 31982, Saudi Arabia)

  • Sherif Mohamed El-Ganainy

    (Department of Arid Land Agriculture, College of Agricultural and Food Sciences, King Faisal University, Hofuf 31982, Saudi Arabia
    Vegetable Diseases Research Department, Plant Pathology Research Institute, Agricultural Research Center (ARC), Giza 12619, Egypt)

Abstract

In 2020, the leaves and fruit of 50 pumpkin plants with suspected cucumber mosaic virus (CMV) symptoms of leaf mosaic, vein yellowing, and mild leaf curling were collected from Sharq El-Owainat (Al Wadi El-Gaded governorate), a new reclamation land in Egypt. This study was aimed at deciphering and characterizing the causal agent of the leaf yellowing disease associated with pumpkin plants in Egypt. The causal agent was identified by serological, cytological, and molecular means. The serological identification by DAS–ELISA confirmed the presence of CMV in 20% of the plants. The cytological identification by electron microscopy revealed typical cucumovirus isometric particles of 28–30 nm diameter in the cytoplasm of the leaf parenchyma, epidermal cells, the integument, and the nucleus. Molecular characterization by one-step reverse transcriptase-PCR yielded the required size of amplicon (678 bp) for CMV. Additionally, mechanical sap inoculation was used to determine the host range and symptomatology of the isolated CMV in seventeen different plant species belonging to six different plant families. CMV replicated, moved systemically, and induced a range of symptoms in sixteen plant species. The isolated CMV was transmitted to pumpkin plants at a 16.4% rate by seeds. CMV-infected pumpkin plant leaves were characterized by a substantially low concentration of photosynthetic pigments, a high level of reducing sugars, relatively low protein levels, and a significant increase in total phenol contents, implying their potential role as antiviral agents. Ultrathin sections of infected cells revealed histological changes and cytological abnormalities in comparison to healthy plants. This is the first identification of CMV on new reclamation land in Egypt, pinpointing its swift spread, which could pose a major constraint to pumpkin production in Egypt.

Suggested Citation

  • Wael Fathy Shehata & Zafar Iqbal & Tarek Elsayed Abdelbaset & Khalied Ibrahiem Saker & Ahmed Elnabawy El Shorbagy & Ahmed Mohamed Soliman & Muhammad Naeem Sattar & Sherif Mohamed El-Ganainy, 2023. "Identification of a Cucumber Mosaic Virus from Cucurbita pepo on New Reclamation Land in Egypt and the Changes Induced in Pumpkin Plants," Sustainability, MDPI, vol. 15(12), pages 1-19, June.
  • Handle: RePEc:gam:jsusta:v:15:y:2023:i:12:p:9751-:d:1174162
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    References listed on IDEAS

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    1. David A. Walker, 1985. "From The Editor," Journal of Financial Research, Southern Finance Association;Southwestern Finance Association, vol. 8(1), pages 1-1, March.
    2. David A. Walker, 1985. "From The Editor," Journal of Financial Research, Southern Finance Association;Southwestern Finance Association, vol. 8(3), pages 1-1, September.
    3. David A. Walker, 1985. "From The Editor," Journal of Financial Research, Southern Finance Association;Southwestern Finance Association, vol. 8(2), pages 1-1, June.
    4. David A. Walker, 1985. "From The Editor," Journal of Financial Research, Southern Finance Association;Southwestern Finance Association, vol. 8(4), pages 1-1, December.
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