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Isolation and Characterization of Three New Crude Oil Degrading Yeast Strains, Candida parapsilosis SK1, Rhodotorula mucilaginosa SK2 and SK3

Author

Listed:
  • Safaâ Benmessaoud

    (Laboratory of Biotechnology, Conservation and Valorisation of Naturals Resources (LBCVNR), Faculty of Sciences Dhar El Mehraz, University Sidi Mohamed Ben Abdallah, BP 1796 Atlas, Fez 30000, Morocco)

  • Jaouad Anissi

    (School of Engineering BIOMEDTECH, Euro-Mediterranean University of Fez, Rond-Point Bensouda, Route de Meknes BP 51, Fez 30070, Morocco)

  • Mohammed Kara

    (Laboratory of Biotechnology, Conservation and Valorisation of Naturals Resources (LBCVNR), Faculty of Sciences Dhar El Mehraz, University Sidi Mohamed Ben Abdallah, BP 1796 Atlas, Fez 30000, Morocco)

  • Amine Assouguem

    (Laboratory of Functional Ecology and Environment, Faculty of Sciences and Technology, Sidi Mohamed Ben Abdellah University, Imouzzer Street, P.O. Box 2202, Fez 30000, Morocco)

  • Arwa Abdulkreem AL-Huqail

    (Department of Biology, College of Science, Princess Nourah Bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia)

  • Mousa O. Germoush

    (Biology Department, College of Science, Jouf University, P.O. Box 2014, Sakaka 72388, Saudi Arabia)

  • Riaz Ullah

    (Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia)

  • Sezai Ercisli

    (Department of Horticulture, Faculty of Agriculture, Ataturk University, Erzurum 25240, Turkey)

  • Jamila Bahhou

    (Laboratory of Biotechnology, Conservation and Valorisation of Naturals Resources (LBCVNR), Faculty of Sciences Dhar El Mehraz, University Sidi Mohamed Ben Abdallah, BP 1796 Atlas, Fez 30000, Morocco)

Abstract

Bioremediation using yeasts is an alternative way to minimize the effects of oil spillage on soil. This paper aims to establish a bioremediation protocol involving the optimization of physicochemical parameters. In this regard, three new yeast strains, SK1, SK2 and SK3, were isolated from hydrocarbon-contaminated samples from the Fez-Meknes region, Morocco. These isolates were identified as new species of Candida parapsilosis (SK1) and Rhodotorula mucilaginosa (SK2 and SK3), respectively, based on the similarity of their ITS region. The kinetic analysis of the process of degradation of petroleum oils are highlighted. These analyses were based on the degradation kinetics, and biomass formation using gravimetric analysis and gas chromatography coupled with mass spectrometry techniques. The strains were able to degrade 68% of the total petroleum hydrocarbon in 21 days, as the sole carbon source. The addition of glucose increased the rate at which crude oil was consumed by the isolates. Our results suggest that inoculants based on Candida parapsilosis (SK1) and Rhodotorula mucilaginosa (SK2 and SK3) cells have potential application in the biodegradation of crude oil and possibly in the degradation of other related aromatic compounds.

Suggested Citation

  • Safaâ Benmessaoud & Jaouad Anissi & Mohammed Kara & Amine Assouguem & Arwa Abdulkreem AL-Huqail & Mousa O. Germoush & Riaz Ullah & Sezai Ercisli & Jamila Bahhou, 2022. "Isolation and Characterization of Three New Crude Oil Degrading Yeast Strains, Candida parapsilosis SK1, Rhodotorula mucilaginosa SK2 and SK3," Sustainability, MDPI, vol. 14(6), pages 1-15, March.
    • Handle: RePEc:gam:jsusta:v:14:y:2022:i:6:p:3465-:d:772213
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