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“Nature-like” Cryoimmobilization of Phototrophic Microorganisms: New Opportunities for Their Long-Term Storage and Sustainable Use

Author

Listed:
  • Olga Senko

    (Faculty of Chemistry, Lomonosov Moscow State University, 119991 Moscow, Russia
    N.M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, 119334 Moscow, Russia)

  • Nikolay Stepanov

    (Faculty of Chemistry, Lomonosov Moscow State University, 119991 Moscow, Russia
    N.M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, 119334 Moscow, Russia)

  • Olga Maslova

    (Faculty of Chemistry, Lomonosov Moscow State University, 119991 Moscow, Russia)

  • Elena Efremenko

    (Faculty of Chemistry, Lomonosov Moscow State University, 119991 Moscow, Russia
    N.M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, 119334 Moscow, Russia)

Abstract

It was found that immobilization of cells in poly(vinyl alcohol) (PVA) cryogel can be successfully applied for concurrent cryoimmobilization, cryoconservation and long-term storage of the cells of various phototrophic microorganisms (green and red microalgae, diatoms and cyanobacteria). For the first time, it was shown for 12 different immobilized microalgal cells that they can be stored frozen for at least 18 months while retaining a high level of viability (90%), and can further be used as an inoculum upon defrosting for cell-free biomass accumulation. Application of cryoimmobilized Chlorella vulgaris cells as inocula allowed the loading of a high concentration of the microalgal cells into the media for free biomass accumulation, thus increasing the rate of the process. It was shown that as minimum of 5 cycles of reuse of the same immobilized cells as inocula for cell accumulation could be realized when various real wastewater samples were applied as media for simultaneous microalgae cultivation and water purification.

Suggested Citation

  • Olga Senko & Nikolay Stepanov & Olga Maslova & Elena Efremenko, 2022. "“Nature-like” Cryoimmobilization of Phototrophic Microorganisms: New Opportunities for Their Long-Term Storage and Sustainable Use," Sustainability, MDPI, vol. 14(2), pages 1-16, January.
    • Handle: RePEc:gam:jsusta:v:14:y:2022:i:2:p:661-:d:719879
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