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Enhancement of Wheat Growth by UV-Mutagenesis of Potential Chromium Tolerant Bacillus sp. Strain SR-2-1/1

Author

Listed:
  • Muhammad Shahid

    (Department of Bioinformatics & Biotechnology, Government College University Faisalabad, Faisalabad 38040, Pakistan)

  • Muhammad Usman

    (Department of Bioinformatics & Biotechnology, Government College University Faisalabad, Faisalabad 38040, Pakistan)

  • Tanvir Shahzad

    (Department of Environmental Sciences, Government College University Faisalabad, Faisalabad 38040, Pakistan)

  • Iftikhar Ali

    (State Key Laboratory of Molecular Development Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences (CAS), Beijing 100101, China
    Center for Plant Sciences and Biodiversity, University of Swat, Charbagh, Swat 19120, Pakistan)

  • Muhammad Umair Hassan

    (Research Center on Ecological Sciences, Jiangxi Agricultural University, Nanchang 330045, China)

  • Faisal Mahmood

    (Department of Environmental Sciences, Government College University Faisalabad, Faisalabad 38040, Pakistan)

  • Sameer H. Qari

    (Department of Biology, Al-Jumum University College, Umm Al-Qura University, Makkah 21955, Saudi Arabia)

Abstract

UV mutagenesis has long been known to improve bacterial strains in their physiological capacity. In the current study, we used the UV mutagenesis approach to increase the PGPR characteristics of a pre-characterized metal-tolerant PGPR strain Bacillus sp. strain SR-2-1/1 (KY315919), with the objective of increasing the physiological outcome of its PGPR traits in vitro and post inoculation on wheat plants. After UV irradiation, the results implied a substantial in vitro increase in the phosphate solubilization and ammonia production of two selected mutant strains (M 1 and M 2 ) as compared to the wild-type strain SR-2-1/1; however, the ACC deaminase enzyme activity was completely lost in the mutant strains, which were originally present in the wild-type strains. However, the UV mutagenesis did not affect the taxonomy of these mutant strains. Moreover, the mutant strains M 1 and M 2 survived in the rhizosphere of wheat plants up to 30 days at an optimum (approximately 7–7.5 Log CFU/mL of rhizosphere soil) population density. The fresh and dry biomass, as well as root and shoot length, of wheat plants inoculated with one of the mutant strains M 2 were significantly higher than in the wheat plants inoculated with wild-type strain SR-2-1/1. The overall results imply that the resulted mutant M 2 was a physiologically competent PGPR strain, which could be tested in field experiments as an inoculum.

Suggested Citation

  • Muhammad Shahid & Muhammad Usman & Tanvir Shahzad & Iftikhar Ali & Muhammad Umair Hassan & Faisal Mahmood & Sameer H. Qari, 2022. "Enhancement of Wheat Growth by UV-Mutagenesis of Potential Chromium Tolerant Bacillus sp. Strain SR-2-1/1," Sustainability, MDPI, vol. 14(22), pages 1-13, November.
    • Handle: RePEc:gam:jsusta:v:14:y:2022:i:22:p:15341-:d:977033
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    References listed on IDEAS

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    1. Muhammad Irfan Ahamad & Jinxi Song & Haotian Sun & Xinxin Wang & Muhammad Sajid Mehmood & Muhammad Sajid & Ping Su & Asif Jamal Khan, 2020. "Contamination Level, Ecological Risk, and Source Identification of Heavy Metals in the Hyporheic Zone of the Weihe River, China," IJERPH, MDPI, vol. 17(3), pages 1-17, February.
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