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The Establishment of Rapid Propagation System of ‘RED SUN’ Phalaenopsis aphrodite

Author

Listed:
  • Haibo Zhang

    (Rare Plants Research Institute of Yangtze River, Three Gorges Corporation, Yichang 443000, China
    National Engineering Research Center of Eco-Environment Protection for Yangtze River Economic Belt National Engineering, Beijing 100000, China)

  • Di He

    (Engineering Research Center of Eco-Environment in Three Gorges Reservoir Region, Ministry of Education, Hubei International Scientific and Technological Center of Ecological Conservation and Management in the Three Gorges Area, China Three Gorges University, Yichang 443002, China)

  • Xiaoling Li

    (Engineering Research Center of Eco-Environment in Three Gorges Reservoir Region, Ministry of Education, Hubei International Scientific and Technological Center of Ecological Conservation and Management in the Three Gorges Area, China Three Gorges University, Yichang 443002, China)

  • Bicheng Dun

    (Rare Plants Research Institute of Yangtze River, Three Gorges Corporation, Yichang 443000, China
    National Engineering Research Center of Eco-Environment Protection for Yangtze River Economic Belt National Engineering, Beijing 100000, China)

  • Di Wu

    (Rare Plants Research Institute of Yangtze River, Three Gorges Corporation, Yichang 443000, China
    National Engineering Research Center of Eco-Environment Protection for Yangtze River Economic Belt National Engineering, Beijing 100000, China)

  • Guiyun Huang

    (Rare Plants Research Institute of Yangtze River, Three Gorges Corporation, Yichang 443000, China
    National Engineering Research Center of Eco-Environment Protection for Yangtze River Economic Belt National Engineering, Beijing 100000, China)

Abstract

Phalaenopsis aphrodite Rchb. F. is a plant of great ornamental and economic value, and its growth has strict requirements in terms of environment. In order to establish the rapid propagation of Phalaenopsis aphrodite Rchb. F., this study used the popular large-flowered variety ‘RED SUN’, which is found on the market, as the material, and studied the effects of pedicel taking time, site, plant growth regulators, and organic substances on the establishment of a regeneration system. The results indicated that the best combination of initiation culture was collecting peduncles in January + upper position of peduncles + 0.1% HgCl 2 sterilization for 8 min. The best recipe for inducing adventitious buds was MS + TDZ 0.4 mg/L + NAA 0.5 mg/L. The optimal combination for adventitious bud propagation was MS + TDZ 0.2 mg/L + NAA 0.4 mg/L. The best recipe for strong seedling from aseptic seedlings was MS + NAA 0.4 mg/L + banana powder 20 g/L + tryptone 2 g. The best recipe for rooting for aseptic seedlings was 1/2 MS + IBA 0.5 mg/L + charcoal 2 g/L. The best combination of acclimation and transplanting for Phalaenopsis was using sphagnum in the training seedling room, which produced a seedling survival rate of 97%. Our findings, therefore, demonstrate the methods of rapid breeding of the Phalaenopsis orchid, which provide a scientific basis for the conservation of this species.

Suggested Citation

  • Haibo Zhang & Di He & Xiaoling Li & Bicheng Dun & Di Wu & Guiyun Huang, 2022. "The Establishment of Rapid Propagation System of ‘RED SUN’ Phalaenopsis aphrodite," Sustainability, MDPI, vol. 14(22), pages 1-14, November.
    • Handle: RePEc:gam:jsusta:v:14:y:2022:i:22:p:15305-:d:976260
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