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Genetic Diversity of Trichoderma harzianum Isolates in Sunflower Rhizosphere: The Application of the URP Molecular Marker

Author

Listed:
  • Younes Rezaee Danesh

    (Department of Plant Protection, Faculty of Agriculture, Urmia University, Urmia 5756151818, Iran)

  • Marika Pellegrini

    (Department of Life, Health and Environmental Sciences, University of L’Aquila, Via Vetoio, 67100 Coppito, Italy)

  • Khalil Kariman

    (UWA School of Agriculture and Environment, The University of Western Australia, Perth 6009, Australia)

  • Gökhan Boyno

    (Department of Plant Protection, Faculty of Agriculture, Van Yuzuncu Yil University, Van 65000, Türkiye)

  • Rihab Djebaili

    (Department of Life, Health and Environmental Sciences, University of L’Aquila, Via Vetoio, 67100 Coppito, Italy)

  • Beatrice Farda

    (Department of Life, Health and Environmental Sciences, University of L’Aquila, Via Vetoio, 67100 Coppito, Italy)

  • Solmaz Najafi

    (Department of Field Crops, Faculty of Agriculture, Van Yuzuncu Yil University, Van 65000, Türkiye)

Abstract

The genetic diversity of 77 Trichoderma harzianum isolates collected from sunflower rhizosphere soils in Urmia, Khoy, and Salmas in West Azerbaijan province, Iran, was evaluated by using the Universal Rice Primer (URP) molecular marker. The DNA band pattern of the isolates was developed using seven primers of this marker. These primers produced 186 gene loci, out of which 182 loci were polymorphic. Accordingly, the genetic diversity of the isolates was calculated, and their kinship relations were determined by cluster analysis using the NTSYS software package. URP-6R had the highest marker index among the studied primers, followed by URP-1F, URP-4R, and URP-25F, implying their higher efficiency in discriminating between the isolates. The results showed that the URP marker could discriminate between isolates using macroscopic morphological characteristics, such as color and colony type, potential of pigment production in the culture medium, and colony growth rate. Furthermore, there was no significant relationship between the geographical distribution of the isolates and the band patterns generated by the primers except for a few cases. The results generally revealed that the URP marker was an efficient tool for determining the genetic diversity of T. harzianum .

Suggested Citation

  • Younes Rezaee Danesh & Marika Pellegrini & Khalil Kariman & Gökhan Boyno & Rihab Djebaili & Beatrice Farda & Solmaz Najafi, 2022. "Genetic Diversity of Trichoderma harzianum Isolates in Sunflower Rhizosphere: The Application of the URP Molecular Marker," Sustainability, MDPI, vol. 14(22), pages 1-12, November.
    • Handle: RePEc:gam:jsusta:v:14:y:2022:i:22:p:15111-:d:973140
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    References listed on IDEAS

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    1. Ruei-Teng Lyu & Cheng-Hua Huang, 2022. "Supplementation of Manure Compost with Trichoderma asperellum Improves the Nutrient Uptake and Yield of Edible Amaranth under Field Conditions," Sustainability, MDPI, vol. 14(9), pages 1-13, April.
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