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Endocrine Disruption, Cytotoxicity and Genotoxicity of an Organophosphorus Insecticide

Author

Listed:
  • Afifa Belaid

    (Research Unit of Analysis and Process Applied to the Environment, Higher Institute of Applied Sciences and Technology, Mahdia 5100, Tunisia)

  • Nosra Methneni

    (Research Unit of Analysis and Process Applied to the Environment, Higher Institute of Applied Sciences and Technology, Mahdia 5100, Tunisia)

  • Emna Nasri

    (Research Unit of Analysis and Process Applied to the Environment, Higher Institute of Applied Sciences and Technology, Mahdia 5100, Tunisia)

  • Sarra Bchir

    (Research Unit of Analysis and Process Applied to the Environment, Higher Institute of Applied Sciences and Technology, Mahdia 5100, Tunisia)

  • Roel Anthonissen

    (Scientific Direction Chemical and Physical Health Risks, Sciensano, 1050 Brussels, Belgium)

  • Luc Verschaeve

    (Scientific Direction Chemical and Physical Health Risks, Sciensano, 1050 Brussels, Belgium)

  • Véronique Le Tilly

    (IRDL FRE CNRS 3744, Université Bretagne Sud, 56100 Lorient, France)

  • Vincenzo Lo Turco

    (BioMorf Department, University of Messina, 98168 Messina, Italy)

  • Giuseppa Di Bella

    (BioMorf Department, University of Messina, 98168 Messina, Italy)

  • Hedi Ben Mansour

    (Research Unit of Analysis and Process Applied to the Environment, Higher Institute of Applied Sciences and Technology, Mahdia 5100, Tunisia)

  • Nezar H. Khdary

    (King Abdulaziz City for Science and Technology (KACST), Riyadh 11442, Saudi Arabia)

Abstract

In the present study, a battery of biological tests undertaken in vitro and in vivo was used to evaluate the toxic potential of an organophosphorus insecticide, namely Fenitrothion. The cytotoxic effect of pesticide was evaluated with the MTT assay against two human cancer cell lines: Hep-2 and MDA-MB-231. Genotoxicity was also studied using the bacterial VITOTOX ® assay. The estrogenic effect was tested using the recombinant yeasts (YES) assay. Likewise, bioluminescence assays using V. fischeri and D. magna immobilization were performed. The results showed that Fenitrothion exhibits a variable cytotoxic effect depending on the dose as well as the studied cell lines, and no genotoxicity was observed in the tested sample. However, an estrogenic effect was recorded when investigating Fenitrothion using the recombinant yeasts (YES) assay. Analogously, acute toxicity was observed for both organisms and at all tested concentrations of Fenitrothion. Overall, these results underline the crucial importance of in vitro and in vivo bioassays in monitoring toxicity of pesticides.

Suggested Citation

  • Afifa Belaid & Nosra Methneni & Emna Nasri & Sarra Bchir & Roel Anthonissen & Luc Verschaeve & Véronique Le Tilly & Vincenzo Lo Turco & Giuseppa Di Bella & Hedi Ben Mansour & Nezar H. Khdary, 2021. "Endocrine Disruption, Cytotoxicity and Genotoxicity of an Organophosphorus Insecticide," Sustainability, MDPI, vol. 13(20), pages 1-13, October.
    • Handle: RePEc:gam:jsusta:v:13:y:2021:i:20:p:11512-:d:659115
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