IDEAS home Printed from https://ideas.repec.org/a/gam/jsusta/v13y2021i18p10072-d631622.html
   My bibliography  Save this article

Isolation, Characterization and Identification of a New Lysinibacillus fusiformis Strain ZC from Metlaoui Phosphate Laundries Wastewater: Bio-Treatment Assays

Author

Listed:
  • Ines Taieb

    (Laboratory of Analysis, Treatment and Valorization of the Pollutants of the Environment and Products, Faculty of Pharmacy, University of Monastir, Avicenne Street, Monastir 5000, Tunisia
    Faculté des Sciences de Gafsa, Campus Universitaire Sidi Ahmed Zarroug, Université de Gafsa, Gafsa 2112, Tunisia)

  • Sonia Ben Younes

    (Faculté des Sciences de Gafsa, Campus Universitaire Sidi Ahmed Zarroug, Université de Gafsa, Gafsa 2112, Tunisia
    Unité de Recherches n°17/ES/13, Faculté de Médecine de Tunis, Université de Tunis El Manar, Tunis 1007, Tunisia)

  • Boutheina Messai

    (Faculté des Sciences de Gafsa, Campus Universitaire Sidi Ahmed Zarroug, Université de Gafsa, Gafsa 2112, Tunisia)

  • Sami Mnif

    (Laboratory of Molecular and Cellular Screening Processes, Centre of Biotechnology of Sfax, P.O. Box 1177, Sfax 3018, Tunisia)

  • Ridha Mzoughi

    (Laboratory of Analysis, Treatment and Valorization of the Pollutants of the Environment and Products, Faculty of Pharmacy, University of Monastir, Avicenne Street, Monastir 5000, Tunisia)

  • Amina Bakhrouf

    (Laboratory of Analysis, Treatment and Valorization of the Pollutants of the Environment and Products, Faculty of Pharmacy, University of Monastir, Avicenne Street, Monastir 5000, Tunisia)

  • Chédia Jabeur

    (Laboratory of Analysis, Treatment and Valorization of the Pollutants of the Environment and Products, Faculty of Pharmacy, University of Monastir, Avicenne Street, Monastir 5000, Tunisia)

  • Juan Alfonso Ayala Serrano

    (Centro de Biologia Molecular “Severo Ochoa”, Campus Cantoblanco, CSIC—Consejo Superior de Investigaciones Científicas, 28049 Madrid, Spain)

  • Ali Ellafi

    (Laboratory of Analysis, Treatment and Valorization of the Pollutants of the Environment and Products, Faculty of Pharmacy, University of Monastir, Avicenne Street, Monastir 5000, Tunisia
    Faculté des Sciences de Gafsa, Campus Universitaire Sidi Ahmed Zarroug, Université de Gafsa, Gafsa 2112, Tunisia)

Abstract

The aim of the present study is to isolate, characterize and identify a novel strain ZC from the Metlaoui phosphate laundries wastewater (MPLW). The chemical characterization of this phosphate rich effluent showed an alkaline pH and is saline, highly turbid and rich in suspended matter and total solids. The MPLW samples were loaded with potentially toxic metals, presented in decreasing order as follows: magnesium (5655 mg L −1 ), potassium (45 mg L −1 ), lead (1 mg L −1 ), iron (0.7 mg L −1 ), cadmium (0.5 mg L −1 ), copper (0.3 mg L −1 ) and zinc (0.1 mg L −1 ). Due to the high COD/BOD 5 ratio, a poorly biodegradable organic load is underlining. The newly isolated strain was identified as Lysinibacillus fusiformis using 16S rDNA sequencing analysis. The viability of this new strain was tested in presence of the zinc, lead, cadmium, manganese and copper at 1, 10 and 100 mM. The L. fusiformis survival, under metallic stress, was inversely proportional to metal ion concentrations, while lead and zinc were the most toxic ones using MTT assay. Then, the newly isolated strain was characterized in terms of enzyme production, proteomic alteration and antibiotic resistance. The strain ZC revealed some modifications in the biochemical and enzymatic profiles by either the appearance or/and the disappearance of some activities. In addition, the increase in metal ions stress and concentrations was proportional to the adherence and to the hydrophobicity. The presence of the metal ions suggested the change of sensitivity to the resistance of this strain towards tobramycin, kanamycin, neomycin, netilmicin and cefoxitin, showing an increase in the MAR index . The strain ZC, used as a biological tool for MPLW treatment, showed a reduction in the metal ion contents. This reduction was due to accumulation and/or adsorption, showing a bioprocessing performance of the newly isolated L. fusiformis .

Suggested Citation

  • Ines Taieb & Sonia Ben Younes & Boutheina Messai & Sami Mnif & Ridha Mzoughi & Amina Bakhrouf & Chédia Jabeur & Juan Alfonso Ayala Serrano & Ali Ellafi, 2021. "Isolation, Characterization and Identification of a New Lysinibacillus fusiformis Strain ZC from Metlaoui Phosphate Laundries Wastewater: Bio-Treatment Assays," Sustainability, MDPI, vol. 13(18), pages 1-19, September.
    • Handle: RePEc:gam:jsusta:v:13:y:2021:i:18:p:10072-:d:631622
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/2071-1050/13/18/10072/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/2071-1050/13/18/10072/
    Download Restriction: no
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Boutheina Messai & Ines Taieb & Sonia Ben Younes & Bruno Lartiges & Ezzedine Ben Salem & Ali Ellafi, 2023. "Characterization of the Tunisian Phosphate Rock from Metlaoui-Gafsa Basin and Bio-Leaching Assays," Sustainability, MDPI, vol. 15(9), pages 1-12, April.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jsusta:v:13:y:2021:i:18:p:10072-:d:631622. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.