Author
Listed:
- Itzel C. Romero-Soto
(Laboratorio de Investigación en Biotecnología, Centro Universitario del Norte, Universidad de Guadalajara, Colotlán 46200, Jalisco, Mexico)
- Raúl B. Martínez-Pérez
(Biotecnología Industrial, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), Zapopan 45019, Jalisco, Mexico)
- Jorge A. Rodríguez
(Biotecnología Industrial, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), Zapopan 45019, Jalisco, Mexico)
- Rosa M. Camacho-Ruiz
(Biotecnología Industrial, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), Zapopan 45019, Jalisco, Mexico)
- Alejandra Barbachano-Torres
(Biotecnología Industrial, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), Zapopan 45019, Jalisco, Mexico)
- Martha Martín del Campo
(Laboratorio de Investigación en Biotecnología, Centro Universitario del Norte, Universidad de Guadalajara, Colotlán 46200, Jalisco, Mexico)
- Juan Napoles-Armenta
(Facultad de Agronomía, Universidad Autónoma de Nuevo León, Francisco I. Madero S/N, Ex Hacienda el Cañada, Gral. Escobedo 66050, Nuevo León, Mexico)
- Jorge E. Pliego-Sandoval
(Laboratorio de Ciencias de la Naturaleza. Centro Universitario del Sur, Av. Enrique Arreola Silva 883, Col Centro, Ciudad Guzmán 49000, Jalisco, Mexico)
- María O. Concha-Guzmán
(Laboratorio de Investigación en Biotecnología, Centro Universitario del Norte, Universidad de Guadalajara, Colotlán 46200, Jalisco, Mexico)
- María Angeles Camacho-Ruiz
(Laboratorio de Investigación en Biotecnología, Centro Universitario del Norte, Universidad de Guadalajara, Colotlán 46200, Jalisco, Mexico)
Abstract
In the present study, a novel laccase from ascomycete Gliomastix murorum was produced in agro-industrial wastes and entrapped in galactomannan beads for Reactive Blue 2 (Rb-2) decolorization. The maximum laccase production in agave bagasse-based medium occurred at 72 h (1798.6 UL −1 ). Entrapped laccase decolorized ˃80% of 0.5 mM Rb-2 in 2 h without the addition of redox mediator. K m for Rb-2 substrate was 1.42 mM, with a V max of 1.19 µmol min −1 for entrapped laccase. Galactomannan matrices produce stability to acid pH (2–5) and temperatures from 20–70 °C. Reusability assays showed that entrapped laccase could retain efficient Rb-2 decolorization of ˃80% six times. In general, galactomannan used for entrapment of laccase provides economic advantages in large-scale wastewater treatment due to its natural origin and efficient results.
Suggested Citation
Itzel C. Romero-Soto & Raúl B. Martínez-Pérez & Jorge A. Rodríguez & Rosa M. Camacho-Ruiz & Alejandra Barbachano-Torres & Martha Martín del Campo & Juan Napoles-Armenta & Jorge E. Pliego-Sandoval & Ma, 2021.
"Galactomannans for Entrapment of Gliomastix murorum Laccase and Their Use in Reactive Blue 2 Decolorization,"
Sustainability, MDPI, vol. 13(16), pages 1-12, August.
Handle:
RePEc:gam:jsusta:v:13:y:2021:i:16:p:9019-:d:612987
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