Author
Listed:
- Dalia Sukmawati
(Biology Department, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta 13220, Indonesia
Universitas Negeri Culture Collection, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta 13220, Indonesia)
- Nurul Family
(Biology Department, Faculty of Mathematics and Natural Sciences, Universitas Negeri Jakarta, Jakarta 13220, Indonesia)
- Iman Hidayat
(Microbiology Division, Research Centre for Biology, Indonesian Institute of Sciences (LIPI), Bogor 16911, Indonesia)
- R. Z. Sayyed
(Department of Microbiology, PSGVP Mandal’s, Arts, Science & Commerce College, Shahada 425409, India)
- Elsayed A. Elsayed
(Bioproducts Research Chair, Zoology Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia
Chemistry of Natural and Microbial Products Department, National Research Centre, Cairo 12622, Egypt)
- Daniel Joe Dailin
(Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM), Skudai 81310, Malaysia
School of Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia, Skudai 81310, Malaysia)
- Siti Zulaiha Hanapi
(Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM), Skudai 81310, Malaysia)
- Mohammad A. Wadaan
(Bioproducts Research Chair, Zoology Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia)
- Hesham El Enshasy
(Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM), Skudai 81310, Malaysia
School of Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia, Skudai 81310, Malaysia
City of Scientific Research and Technology Applications (SRTA), New Burg Al Arab, Alexandria 21934, Egypt)
Abstract
This study aimed to isolate and identify moulds from rotten Citrus sinensis post-harvests and to investigate the activity of antagonist and biocontrol activity moulds that cause citrus fruit rotting. A total of 12 mould isolates were obtained. Following the pathogenicity test, two representative mould isolates were selected and identified based on the sequence analyses of internal transcribed spacer (ITS) regions of the rDNA. Methods used in this study include isolation of fungal postharvest diseases, pathogenicity assay, antagonism assay, growth curve analysis, in vitro biocontrol assay, and molecular phylogenetic analysis. Two isolates of fungal postharvest diseases were determined as the most destructive pathogens. The biocontrol assay showed that isolates of Y1 and Y10 were capable to reduce the growth of fungal isolates K6 and K9 and mitigate up to 100% of the damage of sweet citrus fruits after 7 days of incubation. The moulds were identified as K6 ( Aspergillus flavus sensu lato) and K9 ( Aspergillus niger sensu lato). Phylogenetic analysis showed that the Y10 yeast isolate was identified as Candida orthopsilosis , whereas the Y1 isolate had a close genetic relationship with Aureobasidium pullulans and possibly belongs to a new species. Further analysis is necessary to confirm this finding.
Suggested Citation
Dalia Sukmawati & Nurul Family & Iman Hidayat & R. Z. Sayyed & Elsayed A. Elsayed & Daniel Joe Dailin & Siti Zulaiha Hanapi & Mohammad A. Wadaan & Hesham El Enshasy, 2021.
"Biocontrol Activity of Aureubasidium pullulans and Candida orthopsilosis Isolated from Tectona grandis L. Phylloplane against Aspergillus sp. in Post-Harvested Citrus Fruit,"
Sustainability, MDPI, vol. 13(13), pages 1-15, July.
Handle:
RePEc:gam:jsusta:v:13:y:2021:i:13:p:7479-:d:588567
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