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Linking Organic Metabolites as Produced by Purpureocillium Lilacinum 6029 Cultured on Karanja Deoiled Cake Medium for the Sustainable Management of Root-Knot Nematodes

Author

Listed:
  • Abhishek Sharma

    (Amity Food and Agriculture Foundation, Amity University Uttar Pradesh, Noida 201313, India)

  • Aditi Gupta

    (Centre for Rural Development and Technology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India)

  • Manu Dalela

    (Centre for Rural Development and Technology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India)

  • Satyawati Sharma

    (Centre for Rural Development and Technology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India)

  • R. Z. Sayyed

    (Department of Microbiology, PSGVP Mandal’s Arts, Science, and Commerce College, Maharashtra 425409, India)

  • Hesham Ali El Enshasy

    (Institute of Bioproduct Development (IBD), Universiti Teknologi Malaysia (UTM), Johor Bahru, Johor 81310, Malaysia
    School of Chemical and Energy Engineering, Faculty of Engineering, Universiti Teknologi Malaysia (UTM), Johor Bahru, Johor 81310, Malaysia
    City of Scientific Research and Technology Applications (SRTA), New Burg Al Arab, Alexandria 21934, Egypt)

  • Elsayed Ahmed Elsayed

    (Zoology Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia
    Chemistry of Natural and Microbial Products Department, National Research Centre, Cairo 12622, Egypt)

Abstract

Root-knot nematodes pose a serious threat to agriculture and forest systems, causing significant losses of the crop worldwide. A wide range of chemical nematicides has traditionally been used to manage phyto-nematodes. However, due to their ill effects on the environment, biological control agents (BCAs) like Purpureocillium lilacinum that exhibit antagonistic effects on root-knot nematodes are preferred. The current study focused on identifying nematicidal metabolites produced by the fungus Purpureocillium lilacinum cultivated on akaranja deoiled cake-based liquid medium through bioactivity-guided fractionation against Meloidogyne incognita . Column chromatography of the ethyl acetate extract of fungal filtrate exhibited the most potent fraction (fraction 14–15), giving 94.6% egg mass hatching inhibition on the 5th day and a maximum nematicidal activity of 62% against second-stage juveniles after 48 h at 5000 mg/L. Gas chromatography coupled with mass spectrometry (GC-MS) analysis of this fraction revealed five major compounds, viz., 2-ethyl butyric acid, phenyl ethyl alcohol, benzoic acid, benzene acetic acid, and 3,5-Di-t-butylphenol. These biocompounds have potential biocontrol applications in agriculture, but further in vivo studies are warranted.

Suggested Citation

  • Abhishek Sharma & Aditi Gupta & Manu Dalela & Satyawati Sharma & R. Z. Sayyed & Hesham Ali El Enshasy & Elsayed Ahmed Elsayed, 2020. "Linking Organic Metabolites as Produced by Purpureocillium Lilacinum 6029 Cultured on Karanja Deoiled Cake Medium for the Sustainable Management of Root-Knot Nematodes," Sustainability, MDPI, vol. 12(19), pages 1-9, October.
    • Handle: RePEc:gam:jsusta:v:12:y:2020:i:19:p:8276-:d:425003
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