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Croton argyrophyllus Kunth Essential Oil-Loaded Solid Lipid Nanoparticles: Evaluation of Release Profile, Antioxidant Activity and Cytotoxicity in a Neuroblastoma Cell Line

Author

Listed:
  • Eliana B. Souto

    (Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Coimbra (FFUC), Pólo das Ciências da Saúde, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal
    CEB—Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal)

  • Patricia Severino

    (Laboratory of Nanotechnology and Nanomedicine (LNMED), Institute of Technology and Research (ITP), Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil
    Industrial Biotechnology Program, University of Tiradentes (UNIT), Av. Murilo Dantas 300, Aracaju 49032-490, Brazil
    Tiradentes Institute, 150 Mt Vernon St, Dorchester, MA 02125, USA)

  • Conrado Marques

    (Laboratory of Nanotechnology and Nanomedicine (LNMED), Institute of Technology and Research (ITP), Av. Murilo Dantas, 300, Aracaju 49010-390, Brazil
    Industrial Biotechnology Program, University of Tiradentes (UNIT), Av. Murilo Dantas 300, Aracaju 49032-490, Brazil
    Tiradentes Institute, 150 Mt Vernon St, Dorchester, MA 02125, USA)

  • Luciana N. Andrade

    (Department of Physiology, Federal University of Sergipe, São Cristóvão 49100-000, Brazil)

  • Alessandra Durazzo

    (CREA-Research Centre for Food and Nutrition, Via Ardeatina 546, 00178 Rome, Italy)

  • Massimo Lucarini

    (CREA-Research Centre for Food and Nutrition, Via Ardeatina 546, 00178 Rome, Italy)

  • Atanas G. Atanasov

    (Institute of Neurobiology, Bulgarian Academy of Sciences, 23 Acad. G. Bonchev str., 1113 Sofia, Bulgaria
    Institute of Genetics and Animal Biotechnology, Polish Academy of Sciences, Jastrzębiec, 05-552 Magdalenka, Poland
    Department of Pharmacognosy, University of Vienna, Althanstraße 14, 1090 Vienna, Austria
    Ludwig Boltzmann Institute for Digital Health and Patient Safety, Medical University of Vienna, Spitalgasse 23, 1090 Vienna, Austria)

  • Soukaina El Maimouni

    (Department of Pharmacy, University of Napoli Federico II, Via. D. Montesano 49, 80131 Napoli, Italy)

  • Ettore Novellino

    (Department of Pharmacy, University of Napoli Federico II, Via. D. Montesano 49, 80131 Napoli, Italy)

  • Antonello Santini

    (Department of Pharmacy, University of Napoli Federico II, Via. D. Montesano 49, 80131 Napoli, Italy)

Abstract

The essential oil from Croton argyrophyllus Kunth is known for its antiproliferative, anti-inflammatory, antinociceptive, and anticancer activities, and is recognized as a source of phytochemicals for potential use in pharmaceutic and food sectors. Solid lipid nanoparticles (SLN) have been produced to load Croton argyrophyllus ( CA ) Kunth essential oil ( CA EO) and its antioxidant properties evaluated in vitro as a new approach for the treatment of neurodegenerative diseases. Cetyl palmitate SLN loading CA EO ( CA EO-SLN) with a mean particle size of 201.4 ± 2.3 nm (polydispersity index 0.211) have been produced by hot high-pressure homogenisation. The release of the oil followed the Korsmeyers-Peppas model. The risk of lipid peroxidation has been determined by applying the production of thiobarbituric acid-reactive substances (TBARS) standard assay. The antioxidant activity was determined by the capacity of the antioxidants existing in CA EO to scavenge the stable radical DPPH•. The cytotoxicity of CA Kunth essential oil-loaded SLN ( CA EO-SLN) was evaluated in a human cell line SH-SY5Y (derived from human neuroblastoma) by determining the reduction of the yellow dye 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT). Both free essential oil ( f EO) and loaded essential oil ( CA EO-SLN) were demonstrated to inhibit the Fenton reaction. CA EO-SLN showed DPPH• radical scavenging capacity. The loading of the oil into cetyl palmitate SLN reduced the risk of cytotoxicity.

Suggested Citation

  • Eliana B. Souto & Patricia Severino & Conrado Marques & Luciana N. Andrade & Alessandra Durazzo & Massimo Lucarini & Atanas G. Atanasov & Soukaina El Maimouni & Ettore Novellino & Antonello Santini, 2020. "Croton argyrophyllus Kunth Essential Oil-Loaded Solid Lipid Nanoparticles: Evaluation of Release Profile, Antioxidant Activity and Cytotoxicity in a Neuroblastoma Cell Line," Sustainability, MDPI, vol. 12(18), pages 1-12, September.
    • Handle: RePEc:gam:jsusta:v:12:y:2020:i:18:p:7697-:d:415049
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