IDEAS home Printed from https://ideas.repec.org/a/gam/jsusta/v12y2020i17p6988-d404932.html
   My bibliography  Save this article

Preliminary Studies on Fungal Contamination of Two Rupestrian Churches from Matera (Southern Italy)

Author

Listed:
  • Stefania Mirela Mang

    (School of Agricultural, Forestry, Food and Environmental Sciences (SAFE), University of Basilicata, Viale dell’Ateneo Lucano 10, 85100 Potenza, Italy)

  • Laura Scrano

    (Department of European and Mediterranean Cultures (DICEM), University of Basilicata, Via Lanera, 75100 Matera, Italy)

  • Ippolito Camele

    (School of Agricultural, Forestry, Food and Environmental Sciences (SAFE), University of Basilicata, Viale dell’Ateneo Lucano 10, 85100 Potenza, Italy)

Abstract

The Sassi, a UNESCO World Heritage Site and its rupestrian churches, are richly decorated and visited by thousands of visitors every year. It is important to preserve this heritage which shows signs of deterioration due to abiotic and/or biotic factors. Aiming to carry out in the future an environmental-friendly restoration, a screening of the fungi present on walls and frescoes of two rupestrian churches “ Santa Lucia alle Malve ” and “ La Madonna dei derelitti ” located, respectively, in the “Sasso Caveoso” and in the “Sasso Barisano” was performed. Isolation and characterization of fungal species from investigated sites was carried out. Total genomic DNA (gDNA) was extracted from pure fungal cultures and subsequently utilized in PCRs using primers that amplify a portion of the ribosomal DNA (ITS5/ITS4) or the β-tubulin gene (Bt2a/Bt2b). The amplicons were directly sequenced. Obtained nucleotide sequences were compared to those present in the GenBank (NCBI) showing a very high similarity (99–100%) with the following species: Parengyodontium album , Alternaria alternata , Cladosporium cladosporioides , Lecanicillium psalliotae , Meyerozyma guilliermondii and Botryotrichum atrogriseum . All sequences from this study were deposited in the EMBL database. Detailed knowledge about fungi isolated from stone is indispensable not only to counter/reduce the structural and aesthetic damage but also to protect the health of both guardians and visitors who may develop different pathologies due to the spores diffused in the environment.

Suggested Citation

  • Stefania Mirela Mang & Laura Scrano & Ippolito Camele, 2020. "Preliminary Studies on Fungal Contamination of Two Rupestrian Churches from Matera (Southern Italy)," Sustainability, MDPI, vol. 12(17), pages 1-13, August.
    • Handle: RePEc:gam:jsusta:v:12:y:2020:i:17:p:6988-:d:404932
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/2071-1050/12/17/6988/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/2071-1050/12/17/6988/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Giulia Caneva & Maria Rosaria Fidanza & Chiara Tonon & Sergio Enrico Favero-Longo, 2020. "Biodeterioration Patterns and Their Interpretation for Potential Applications to Stone Conservation: A Hypothesis from Allelopathic Inhibitory Effects of Lichens on the Caestia Pyramid (Rome)," Sustainability, MDPI, vol. 12(3), pages 1-15, February.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Florin Marcu & Nicolaie Hodor & Liliana Indrie & Paula Dejeu & Marin Ilieș & Adina Albu & Mircea Sandor & Cosmin Sicora & Monica Costea & Dorina Camelia Ilieș & Tudor Caciora & Anca Huniadi & Iuliana , 2021. "Microbiological, Health and Comfort Aspects of Indoor Air Quality in a Romanian Historical Wooden Church," IJERPH, MDPI, vol. 18(18), pages 1-18, September.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.

      Corrections

      All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jsusta:v:12:y:2020:i:17:p:6988-:d:404932. See general information about how to correct material in RePEc.

      If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

      If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

      If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

      For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

      Please note that corrections may take a couple of weeks to filter through the various RePEc services.

      IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.