IDEAS home Printed from https://ideas.repec.org/a/gam/jijerp/v20y2023i5p3917-d1077076.html
   My bibliography  Save this article

The Identification of Potential Immunogenic Antigens in Particular Active Developmental Stages of the Rice Weevil ( Sitophilus oryzae )

Author

Listed:
  • Joanna Witecka

    (Department of Parasitology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia, 40-055 Katowice, Poland)

  • Natalia Malejky-Kłusek

    (Chair of Ecology and Environmental Protection, Institute of Biology, Pedagogical University of Cracow, Podchorążych 2 St., 30-084 Cracow, Poland)

  • Krzysztof Solarz

    (Department of Parasitology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia, 40-055 Katowice, Poland)

  • Olga Pawełczyk

    (Department of Parasitology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia, 40-055 Katowice, Poland)

  • Małgorzata Kłyś

    (Chair of Ecology and Environmental Protection, Institute of Biology, Pedagogical University of Cracow, Podchorążych 2 St., 30-084 Cracow, Poland)

  • Aleksandra Izdebska

    (Chair of Ecology and Environmental Protection, Institute of Biology, Pedagogical University of Cracow, Podchorążych 2 St., 30-084 Cracow, Poland)

  • Weronika Maślanko

    (Department of Animal Ethology and Wildlife Management, Faculty of Animal Sciences and Bioeconomy, University of Life Sciences in Lublin, Akademicka 13 St., 20-950 Lublin, Poland)

  • Marek Asman

    (Department of Medical and Molecular Biology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia, 40-055 Katowice, Poland)

Abstract

Background: The rice weevil ( Sitophilus oryzae ) originates from subtropical and tropical areas of Asia and Africa, but it also appears on other continents, mostly as a result of trade in rice. It may occur in grain fields as well as in storage facilities, and cause allergenic reactions. The aim of this study was to identify the potential antigens in all developmental stages of S. oryzae, which may cause an allergic response in humans. Methods: Sera of 30 patients were tested for the presence of IgE antibodies to antigens from three life stages of the rice weevil. To identify protein fractions containing potential allergens, proteins collected from larvae, pupae, and adults separated by sex of S. oryzae were fractionated by SDS-PAGE. Then, they were probed with anti-human, anti-IgE monoclonal antibodies, fractionated by SDS-PAGE and detected by Western blotting. Results: In total, 26 protein fractions of males and 22 fractions of other life stages of S. oryzae (larvae, pupae, and females) positively reacted with the examined sera. Conclusions: The conducted study showed that S. oryzae may be a source of many antigens which may cause the potential allergic reactions in humans.

Suggested Citation

  • Joanna Witecka & Natalia Malejky-Kłusek & Krzysztof Solarz & Olga Pawełczyk & Małgorzata Kłyś & Aleksandra Izdebska & Weronika Maślanko & Marek Asman, 2023. "The Identification of Potential Immunogenic Antigens in Particular Active Developmental Stages of the Rice Weevil ( Sitophilus oryzae )," IJERPH, MDPI, vol. 20(5), pages 1-7, February.
    • Handle: RePEc:gam:jijerp:v:20:y:2023:i:5:p:3917-:d:1077076
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/1660-4601/20/5/3917/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/1660-4601/20/5/3917/
    Download Restriction: no
    ---><---

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jijerp:v:20:y:2023:i:5:p:3917-:d:1077076. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.