IDEAS home Printed from https://ideas.repec.org/a/gam/jijerp/v19y2022i7p4112-d783602.html
   My bibliography  Save this article

Tolerance of Human Fibroblasts to Benfo-Oxythiamine In Vitro

Author

Listed:
  • Ming Yan

    (Department of Oral and Maxillofacial Surgery, Guiyang Hospital of Stomatology, Guiyang 550002, China
    Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany)

  • Ralf Smeets

    (Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany
    Department of Oral and Maxillofacial Surgery, Division of “Regenerative Orofacial Medicine”, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany)

  • Martin Gosau

    (Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany)

  • Tobias Vollkommer

    (Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany)

  • Sandra Fuest

    (Department of Oral and Maxillofacial Surgery, Division of “Regenerative Orofacial Medicine”, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany)

  • Eva Stetzer

    (Benfovir AG, 64293 Darmstadt, Germany)

  • Lan Kluwe

    (Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany)

  • Johannes F. Coy

    (Benfovir AG, 64293 Darmstadt, Germany)

  • Simon Burg

    (Department of Oral and Maxillofacial Surgery, University Medical Center Hamburg-Eppendorf, 20246 Hamburg, Germany)

Abstract

Objectives: To explore the potential application of B-OT in the aspiration tract. Materials and Methods: We conceived and optimized an in vitro model simulating the mouth-washing process to assess tolerance to B-OT on primary human gingival fibroblasts. Cells derived from 4 unrelated donors were flushed with medium containing drugs of various concentration for one minute twice daily for 3 days. Results: No effect was seen on the cells up to 1000 µM B-OT. In addition, we treated the cells with B-OT permanently in medium, corresponding to a systemic treatment. No effect was seen by 10 µM B-OT and only a slight reduction (approximately 10%) was seen by 100 µM B-OT. Conclusions: Our results suggest good tolerance of oral cells for B-OT, favoring the further development of this antiviral reagent as a mouth-washing solution and nasal spray.

Suggested Citation

  • Ming Yan & Ralf Smeets & Martin Gosau & Tobias Vollkommer & Sandra Fuest & Eva Stetzer & Lan Kluwe & Johannes F. Coy & Simon Burg, 2022. "Tolerance of Human Fibroblasts to Benfo-Oxythiamine In Vitro," IJERPH, MDPI, vol. 19(7), pages 1-9, March.
    • Handle: RePEc:gam:jijerp:v:19:y:2022:i:7:p:4112-:d:783602
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/1660-4601/19/7/4112/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/1660-4601/19/7/4112/
    Download Restriction: no
    ---><---

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jijerp:v:19:y:2022:i:7:p:4112-:d:783602. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.