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The Impact of Background-Level Carboxylated Single-Walled Carbon Nanotubes (SWCNTs−COOH) on Induced Toxicity in Caenorhabditis elegans and Human Cells

Author

Listed:
  • Jian-He Lu

    (Emerging Compounds Research Center, General Research Service Center, National Pingtung University of Science and Technology, Pingtung 912, Taiwan)

  • Wen-Che Hou

    (Department of Environmental Engineering, National Cheng Kung University, Tainan 701, Taiwan
    These authors contributed equally to this work.)

  • Ming-Hsien Tsai

    (Department of Child Care, College of Humanities and Social Sciences, National Pingtung University of Science and Technology, Pingtung 912, Taiwan
    Research Institute for Life Support Innovation, Research Organization for Nano & Life Innovation, Waseda University, 2-2 Wakamatsucho, Shinjuku, Tokyo 162-8480, Japan)

  • Yu-Ting Chang

    (Department of Environmental Science and Engineering, College of Engineering, National Pingtung University of Science and Technology, Pingtung 912, Taiwan)

  • How-Ran Chao

    (Emerging Compounds Research Center, General Research Service Center, National Pingtung University of Science and Technology, Pingtung 912, Taiwan
    Department of Environmental Science and Engineering, College of Engineering, National Pingtung University of Science and Technology, Pingtung 912, Taiwan
    Institute of Food Safety Management, College of Agriculture, National Pingtung University of Science and Technology, Pingtung 912, Taiwan
    School of Dentistry, College of Dental Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan)

Abstract

Single-walled carbon nanotubes (SWCNTs) are widely utilized for industrial, biomedical, and environmental purposes. The toxicity of Carboxylated SWCNTs (SWCNTs−COOH) in in vivo models, particularly Caenorhabditis elegans ( C. elegans ), and in vitro human cells is still unclear. In this study, C. elegans was used to study the effects of SWCNTs−COOH on lethality, lifespan, growth, reproduction, locomotion, reactive oxygen species (ROS) generation, and the antioxidant system. Our data show that exposure to ≥1 μg·L −1 SWCNTs−COOH could induce toxicity in nematodes that affects lifespan, growth, reproduction, and locomotion behavior. Moreover, the exposure of nematodes to SWCNTs−COOH induced ROS generation and the alteration of antioxidant gene expression. SWCNTs−COOH induced nanotoxic effects at low dose of 0.100 or 1.00 μg·L −1 , particularly for the expression of antioxidants (SOD-3, CTL-2 and CYP-35A2). Similar nanotoxic effects were found in human cells. A low dose of SWCNTs−COOH induced ROS generation and increased the expression of catalase, MnSOD, CuZnSOD, and SOD-2 mRNA but decreased the expression of GPX-2 and GPX-3 mRNA in human monocytes. These findings reveal that background-level SWCNTs−COOH exerts obvious adverse effects, and C. elegans is a sensitive in vivo model that can be used for the biological evaluation of the toxicity of nanomaterials.

Suggested Citation

  • Jian-He Lu & Wen-Che Hou & Ming-Hsien Tsai & Yu-Ting Chang & How-Ran Chao, 2022. "The Impact of Background-Level Carboxylated Single-Walled Carbon Nanotubes (SWCNTs−COOH) on Induced Toxicity in Caenorhabditis elegans and Human Cells," IJERPH, MDPI, vol. 19(3), pages 1-18, January.
    • Handle: RePEc:gam:jijerp:v:19:y:2022:i:3:p:1218-:d:730882
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    Cited by:

    1. Qin Wang & Yi Zhu & Bin Song & Rong Fu & Yanfeng Zhou, 2022. "The In Vivo Toxicity Assessments of Water-Dispersed Fluorescent Silicon Nanoparticles in Caenorhabditis elegans," IJERPH, MDPI, vol. 19(7), pages 1-13, March.

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