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In Vitro Antitumor Activity of Endophytic and Rhizosphere Gram-Positive Bacteria from Ibervillea sonorae (S. Watson) Greene against L5178Y-R Lymphoma Cells

Author

Listed:
  • Ricardo Romero-Arguelles

    (Departamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza 66455, Mexico)

  • César Iván Romo-Sáenz

    (Departamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza 66455, Mexico)

  • Karla Morán-Santibáñez

    (Departamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza 66455, Mexico)

  • Patricia Tamez-Guerra

    (Departamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza 66455, Mexico)

  • Ramiro Quintanilla-Licea

    (Departamento de Química, Universidad Autónoma de Nuevo León, San Nicolás de los Garza 66455, Mexico)

  • Alonso Alberto Orozco-Flores

    (Departamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza 66455, Mexico)

  • Jesica María Ramírez-Villalobos

    (Departamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza 66455, Mexico)

  • Reyes Tamez-Guerra

    (Departamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza 66455, Mexico)

  • Cristina Rodríguez-Padilla

    (Departamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza 66455, Mexico)

  • Ricardo Gomez-Flores

    (Departamento de Microbiología e Inmunología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza 66455, Mexico)

Abstract

Plant-associated microorganisms represent a potential source of new antitumor compounds. The aim of the present study was to isolate endophytic and rhizosphere Gram-positive bacteria from Ibervillea sonorae and produce extracts with antitumor activity. Methanol and ethyl acetate extracts were obtained from 28 d bacterial fermentation, after which murine L5178Y-R lymphoma cells growth inhibition was evaluated at concentrations ranging from 15.62 µg/mL to 500 µg/mL by the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide reduction colorimetric assay. IC 50 and the selectivity index (SI) were calculated and compared with healthy control human peripheral blood mononuclear cells (PBMC). Identification of the isolated strains was performed using the 16S ribosomal gene and by MALDI-TOF MS mass spectrometry. The endophytic and rhizosphere bacterial extracts from strains ISE-B22, ISE-B26, ISE-B27, ISS-A01, ISS-A06, and ISS-A16 showed significant ( p < 0.05) L5178Y-R cell growth inhibition, compared with an untreated control. The rhizosphere Micromonospora echinospora isolate ISS-A16 showed the highest (90.48%) percentage of lymphoma cells growth inhibition and SI (19.1) for PBMC, whereas the Bacillus subtilis ISE-B26 isolate caused significant ( p < 0.01) growth inhibition (84.32%) and a SI of 5.2. Taken together, results of the present study evidenced antitumor effects by I. sonorae endophytic and rhizosphere bacteria culture extracts. Further research will involve the elucidation of the compounds that exert the antitumor activity and their evaluation in pre-clinical studies.

Suggested Citation

  • Ricardo Romero-Arguelles & César Iván Romo-Sáenz & Karla Morán-Santibáñez & Patricia Tamez-Guerra & Ramiro Quintanilla-Licea & Alonso Alberto Orozco-Flores & Jesica María Ramírez-Villalobos & Reyes Ta, 2022. "In Vitro Antitumor Activity of Endophytic and Rhizosphere Gram-Positive Bacteria from Ibervillea sonorae (S. Watson) Greene against L5178Y-R Lymphoma Cells," IJERPH, MDPI, vol. 19(2), pages 1-11, January.
    • Handle: RePEc:gam:jijerp:v:19:y:2022:i:2:p:894-:d:724473
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    References listed on IDEAS

    as
    1. Jesica M. Ramírez-Villalobos & César I. Romo-Sáenz & Karla S. Morán-Santibañez & Patricia Tamez-Guerra & Ramiro Quintanilla-Licea & Alonso A. Orozco-Flores & Ricardo Romero-Arguelles & Reyes Tamez-Gue, 2021. "In Vitro Tumor Cell Growth Inhibition Induced by Lophocereus marginatus (DC.) S. Arias and Terrazas Endophytic Fungi Extracts," IJERPH, MDPI, vol. 18(18), pages 1-10, September.
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    Cited by:

    1. Jesica María Ramírez-Villalobos & Ricardo Gomez-Flores & Priscilla Viridiana Velázquez-Flores & Karla Selene Morán-Santibáñez & Patricia Tamez-Guerra & Orquídea Pérez-González & Myriam Angélica de la , 2023. "Effect of Culture Conditions of Lophocereus marginatus Endophytic Fungi on Yield and Anticancer and Antioxidant Activities," IJERPH, MDPI, vol. 20(5), pages 1-11, February.

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