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The Role of Platelet-Rich Plasma on the Chondrogenic and Osteogenic Differentiation of Human Amniotic-Fluid-Derived Stem Cells

Author

Listed:
  • Alessio Giannetti

    (Department of Life, Health and Environmental Sciences, University of L’Aquila, 67100 L’Aquila, Italy)

  • Andrea Pantalone

    (Clinic of Orthopaedics and Traumatology, “SS. Annunziata” Hospital, 66100 Chieti, Italy)

  • Ivana Antonucci

    (Center of Advanced Studies and Technology (CAST), “G. D’Annunzio” University of Chieti-Pescara, 66100 Chieti, Italy)

  • Sandra Verna

    (Immunohematology and Transfusional Medicine Service, “SS. Annunziata” Hospital, 66100 Chieti, Italy)

  • Patrizia Di Gregorio

    (Immunohematology and Transfusional Medicine Service, “SS. Annunziata” Hospital, 66100 Chieti, Italy)

  • Liborio Stuppia

    (Center of Advanced Studies and Technology (CAST), “G. D’Annunzio” University of Chieti-Pescara, 66100 Chieti, Italy)

  • Vittorio Calvisi

    (Department of Life, Health and Environmental Sciences, University of L’Aquila, 67100 L’Aquila, Italy)

  • Roberto Buda

    (Clinic of Orthopaedics and Traumatology, “SS. Annunziata” Hospital, 66100 Chieti, Italy
    Department of Medicine and Aging Sciences, “G. D’Annunzio” University of Chieti-Pescara, 66100 Chieti, Italy)

  • Vincenzo Salini

    (Department of Orthopaedics and Traumatology, San Raffaele Hospital, 20132 Milan, Italy)

Abstract

Amniotic fluid represents a new and promising source of engraftable stem cells. The purpose of this study was to investigate the in vitro effects of platelet-rich plasma (PRP) on amniotic-fluid-derived stem cells (AFSCs) on chondrogenic or osteogenic differentiation potential. Amniotic fluid samples were obtained from women undergoing amniocentesis for prenatal diagnosis at 16–18 weeks of pregnancy. Undifferentiated human AFSCs were cocultured with PRP for 14 days. The study includes two protocols investigating the effects of activated PRP using two different methods: via freeze–thaw cycles and via the addition of calcium gluconate. On the 14th day of culturing, the differentiation potential of the cocultured AFSCs was then compared with undifferentiated AFSCs. Staining with alcian blue solution (ABS) and alizarine red solution (ARS) was performed, and chondrogenic- and osteogenic-associated genes markers were investigated. ABS demonstrated enhanced glycosaminoglycan expression. Cocultured cells expressed chondrocyte-associated genes, determined by real-time polymerase chain reaction (RT-PCR), including type I collagen, type II collagen, COMP, and aggrecan. In regard to the osteogenic markers, osteopontin and bone sialoprotein, there were no changes. In particular, the activation of PRP using the freeze–thaw cycle protocol showed a higher expression of the chondrogenic markers. Our preliminary in vitro results showed that PRP has good potential in the chondrogenic differentiation of AFSCs.

Suggested Citation

  • Alessio Giannetti & Andrea Pantalone & Ivana Antonucci & Sandra Verna & Patrizia Di Gregorio & Liborio Stuppia & Vittorio Calvisi & Roberto Buda & Vincenzo Salini, 2022. "The Role of Platelet-Rich Plasma on the Chondrogenic and Osteogenic Differentiation of Human Amniotic-Fluid-Derived Stem Cells," IJERPH, MDPI, vol. 19(23), pages 1-12, November.
    • Handle: RePEc:gam:jijerp:v:19:y:2022:i:23:p:15786-:d:985731
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    1. Karol Szyluk & Paweł Niemiec & Dominik Sieroń & Dawid Lukoszek & Marcin Gierek & Andrzej Lorek & Andreas Christe, 2022. "Shoulder Dislocation Incidence and Risk Factors—Rural vs. Urban Populations of Poland," IJERPH, MDPI, vol. 19(19), pages 1-15, September.
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