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Differential Gene Expression and Metabolic Pathway Analysis of Cladophora rupestris under Pb Stress Conditions

Author

Listed:
  • Lei Liu

    (Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China)

  • Lusheng Zhang

    (Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China)

  • Lingyun Zhao

    (Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China)

  • Qiuyu Chen

    (Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China)

  • Qian Zhang

    (Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China)

  • Deju Cao

    (Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China)

  • Zhaowen Liu

    (Anhui Province Key Laboratory of Farmland Ecological Conservation and Pollution Prevention, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China
    School of Materials and Environmental Engineering, Chizhou University, Chizhou 247000, China)

Abstract

This study aimed to analyze the transcriptome of C. rupestris under Pb 2+ stress by using high-throughput sequencing technology, observe the changes of gene expression and metabolic pathway after three and five days under 1.0 and 5.0 mg/L of Pb 2+ treatment, and analyze the differentially expressed genes (DEGs) and related functional genes after Pb 2+ treatment. Metabolic pathways were revealed through Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Results show that DEGs increased significantly with the increase of Pb 2+ concentration and stress time. A total of 32 genes were closely related to Pb 2+ stress response. GO analysis identified two major transporter proteins, namely, ATP-binding transport protein-related (ABC transporters) and zinc finger CCHC domain containing protein (Zfp) in C. rupestris . Pthr19248, pthr19211, Zfp pthr23002, Zfp p48znf pthr12681, Zfp 294 pthr12389, and Zfp pthr23067 played important roles against Pb 2+ toxicity and its absorption in C. rupestris . KEGG pathway analysis suggested that ABCA1, ATM, and ABCD3 were closely related to Pb 2+ absorption. Pb 2+ stress was mainly involved in metallothionein (MT), plant hormone signal transduction, ABC transporters, and glutathione (GSH) metabolism.

Suggested Citation

  • Lei Liu & Lusheng Zhang & Lingyun Zhao & Qiuyu Chen & Qian Zhang & Deju Cao & Zhaowen Liu, 2022. "Differential Gene Expression and Metabolic Pathway Analysis of Cladophora rupestris under Pb Stress Conditions," IJERPH, MDPI, vol. 19(21), pages 1-14, October.
    • Handle: RePEc:gam:jijerp:v:19:y:2022:i:21:p:13910-:d:953570
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