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Quantification of Plasma 8-Isoprostane by High-Performance Liquid Chromatography with Tandem Mass Spectrometry in a Case-Control Study of Lung Cancer

Author

Listed:
  • Lin Ma

    (State Environmental Protection Key Lab of Environmental Risk Assessment and Control on Chemical Processes, School of Resources & Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China
    Department of Public Health Sciences, Pennsylvania State College of Medicine, Hershey, PA 17033, USA)

  • Dongxiao Sun

    (Department of Pharmacology, Mass Spectrometry Core Facility, The Pennsylvania State College of Medicine, Hershey, PA 17033, USA)

  • Guangli Xiu

    (State Environmental Protection Key Lab of Environmental Risk Assessment and Control on Chemical Processes, School of Resources & Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China)

  • Philip Lazarus

    (Department of Pharmaceutical Sciences, College of Pharmacy and Pharmaceutical Sciences, Washington State University, Spokane, WA 99210, USA)

  • Anil Vachani

    (Department of Medicine, Pulmonary, Allergy, and Critical Care Division, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA)

  • Trevor M. Penning

    (Department of Systems Pharmacology & Translational Therapeutics, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA)

  • Alexander S. Whitehead

    (Department of Pharmacology, Center for Pharmacogenetics, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA)

  • Joshua E. Muscat

    (Department of Public Health Sciences, Pennsylvania State College of Medicine, Hershey, PA 17033, USA)

Abstract

Aim: 8-iso-prostaglandin F2α is a biomarker of lipid peroxidation, and one of the most commonly used measures of oxidative stress. It is an established biomarker of lung cancer risk. It is commonly measured by enzyme-linked immunosorbent assay. Given its importance, we developed a stable isotope dilution UPLC-tandem mass spectrometric method for the rapid determination of 8-isoprostane in blood. Methods: We tested the discriminatory capability of the method in 49 lung cancer patients, 55 benign lung nodule patients detected by chest X-ray, and 41 patients with chronic obstructive pulmonary disease (COPD) or asthma. Results: Significant differences were found in mean 8-isoprostane levels between the three groups ( p = 0.027), and post-hoc tests found higher levels in the lung cancer patients than in patients with benign nodules ( p = 0.032) and COPD/asthma ( p = 0.014). The receiving operating characteristic area under the curve (AUC) was 0.69 for differentiating the lung cancer group from the benign nodule group, and 0.7 for differentiating from the COPD/asthma group. Conclusions: The UPLC-MS/MS-based method is an efficient analytical tool for measuring 8-isoprostane plasma concentrations. The results suggest exploring its utility as a marker for early lung cancer screening.

Suggested Citation

  • Lin Ma & Dongxiao Sun & Guangli Xiu & Philip Lazarus & Anil Vachani & Trevor M. Penning & Alexander S. Whitehead & Joshua E. Muscat, 2022. "Quantification of Plasma 8-Isoprostane by High-Performance Liquid Chromatography with Tandem Mass Spectrometry in a Case-Control Study of Lung Cancer," IJERPH, MDPI, vol. 19(19), pages 1-13, September.
    • Handle: RePEc:gam:jijerp:v:19:y:2022:i:19:p:12488-:d:930946
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