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Colorimetric Approach for Nucleic Acid Salmonella spp. Detection: A Systematic Review

Author

Listed:
  • Asma Nadia Ahmad Faris

    (Institute for Research in Molecular Medicine (INFORMM), Health Campus, Universiti Sains Malaysia, Kubang Kerian 16150, Kelantan, Malaysia)

  • Mohamad Ahmad Najib

    (Institute for Research in Molecular Medicine (INFORMM), Health Campus, Universiti Sains Malaysia, Kubang Kerian 16150, Kelantan, Malaysia)

  • Muhammad Najmi Mohd Nazri

    (Institute for Research in Molecular Medicine (INFORMM), Health Campus, Universiti Sains Malaysia, Kubang Kerian 16150, Kelantan, Malaysia)

  • Amir Syahir Amir Hamzah

    (Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia)

  • Ismail Aziah

    (Institute for Research in Molecular Medicine (INFORMM), Health Campus, Universiti Sains Malaysia, Kubang Kerian 16150, Kelantan, Malaysia)

  • Nik Yusnoraini Yusof

    (Institute for Research in Molecular Medicine (INFORMM), Health Campus, Universiti Sains Malaysia, Kubang Kerian 16150, Kelantan, Malaysia)

  • Rohimah Mohamud

    (Department of Immunology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian 16150, Kelantan, Malaysia)

  • Irneza Ismail

    (Advanced Devices & System (ADS) Research Group, Department of Electrical & Electronic Engineering, Faculty of Engineering and Built Environment, Universiti Sains Islam Malaysia, Bandar Baru Nilai, Nilai 71800, Negeri Sembilan, Malaysia)

  • Fatin Hamimi Mustafa

    (Institute for Research in Molecular Medicine (INFORMM), Health Campus, Universiti Sains Malaysia, Kubang Kerian 16150, Kelantan, Malaysia)

Abstract

Water- and food-related health issues have received a lot of attention recently because food-poisoning bacteria, in particular, are becoming serious threats to human health. Currently, techniques used to detect these bacteria are time-consuming and laborious. To overcome these challenges, the colorimetric strategy is attractive because it provides simple, rapid and accurate sensing for the detection of Salmonella spp. bacteria. The aim of this study is to review the progress regarding the colorimetric method of nucleic acid for Salmonella detection. A literature search was conducted using three databases (PubMed, Scopus and ScienceDirect). Of the 88 studies identified in our search, 15 were included for further analysis. Salmonella bacteria from different species, such as S. Typhimurium, S. Enteritidis, S. Typhi and S. Paratyphi A, were identified using the colorimetric method. The limit of detection (LoD) was evaluated in two types of concentrations, which were colony-forming unit (CFU) and CFU per mL. The majority of the studies used spiked samples (53%) rather than real samples (33%) to determine the LoDs. More research is needed to assess the sensitivity and specificity of colorimetric nucleic acid in bacterial detection, as well as its potential use in routine diagnosis.

Suggested Citation

  • Asma Nadia Ahmad Faris & Mohamad Ahmad Najib & Muhammad Najmi Mohd Nazri & Amir Syahir Amir Hamzah & Ismail Aziah & Nik Yusnoraini Yusof & Rohimah Mohamud & Irneza Ismail & Fatin Hamimi Mustafa, 2022. "Colorimetric Approach for Nucleic Acid Salmonella spp. Detection: A Systematic Review," IJERPH, MDPI, vol. 19(17), pages 1-11, August.
    • Handle: RePEc:gam:jijerp:v:19:y:2022:i:17:p:10570-:d:896891
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