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Sub-Inhibitory Concentrations of Amoxicillin and Tylosin Affect the Biofilm Formation and Virulence of Streptococcus suis

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Listed:
  • Jing Zuo

    (College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471000, China
    Key Laboratory of Molecular Pathogen and Immunology of Animal of Luoyang, Luoyang 471000, China)

  • Qingying Fan

    (College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471000, China
    Key Laboratory of Molecular Pathogen and Immunology of Animal of Luoyang, Luoyang 471000, China)

  • Jinpeng Li

    (College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471000, China
    Key Laboratory of Molecular Pathogen and Immunology of Animal of Luoyang, Luoyang 471000, China)

  • Baobao Liu

    (College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471000, China
    Key Laboratory of Molecular Pathogen and Immunology of Animal of Luoyang, Luoyang 471000, China)

  • Bingqian Xue

    (College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471000, China
    Key Laboratory of Molecular Pathogen and Immunology of Animal of Luoyang, Luoyang 471000, China)

  • Xiaoling Zhang

    (College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471000, China
    Key Laboratory of Molecular Pathogen and Immunology of Animal of Luoyang, Luoyang 471000, China)

  • Li Yi

    (Key Laboratory of Molecular Pathogen and Immunology of Animal of Luoyang, Luoyang 471000, China
    College of Life Science, Luoyang Normal University, Luoyang 471000, China)

  • Yang Wang

    (College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471000, China
    Key Laboratory of Molecular Pathogen and Immunology of Animal of Luoyang, Luoyang 471000, China)

Abstract

Streptococcus suis ( S. suis ) can form a protective biofilm during infection and lead to prolonged disease. Oral antibiotics are often used for treatment in clinical practice, but sub-inhibitory concentration levels often exist due to low oral absorption rate, resulting in disease deterioration. The purpose of this study was to investigate the effects of Amoxicillin and Tylosin on the biofilm formation and virulence of S. suis HA9801 at sub-inhibitory concentration. We first determined that the test groups (1/4MIC Amoxicillin and Tylosin) could significantly increase the amount of biofilm formation without affecting bacterial growth. The LD50 value of the test groups was significantly higher than that of the control group in the mouse infection model. In the mouse infection model, the LD50 value of the experimental group was significantly increased, but the tissue bacterial load was significantly decreased. Further RT-PCR analysis showed that the expression levels of virulence-related genes in the experimental group were significantly reduced. Our study suggests that both Amoxicillin and Tylosin at sub-inhibitory concentrations could enhance the biofilm formation ability of S. suis HA9801 and reduce its virulence to form persistent infection.

Suggested Citation

  • Jing Zuo & Qingying Fan & Jinpeng Li & Baobao Liu & Bingqian Xue & Xiaoling Zhang & Li Yi & Yang Wang, 2022. "Sub-Inhibitory Concentrations of Amoxicillin and Tylosin Affect the Biofilm Formation and Virulence of Streptococcus suis," IJERPH, MDPI, vol. 19(14), pages 1-12, July.
    • Handle: RePEc:gam:jijerp:v:19:y:2022:i:14:p:8359-:d:858493
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