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Xylo-Oligosaccharides in Prevention of Hepatic Steatosis and Adipose Tissue Inflammation: Associating Taxonomic and Metabolomic Patterns in Fecal Microbiomes with Biclustering

Author

Listed:
  • Jukka Hintikka

    (Faculty of Sport and Health Sciences, University of Jyväskylä, FI-40014 Jyväskylä, Finland)

  • Sanna Lensu

    (Faculty of Sport and Health Sciences, University of Jyväskylä, FI-40014 Jyväskylä, Finland
    Department of Psychology, University of Jyväskylä, FI-40014 Jyväskylä, Finland)

  • Elina Mäkinen

    (Faculty of Sport and Health Sciences, University of Jyväskylä, FI-40014 Jyväskylä, Finland)

  • Sira Karvinen

    (Faculty of Sport and Health Sciences, University of Jyväskylä, FI-40014 Jyväskylä, Finland)

  • Marjaana Honkanen

    (Faculty of Sport and Health Sciences, University of Jyväskylä, FI-40014 Jyväskylä, Finland)

  • Jere Lindén

    (Veterinary Pathology and Parasitology and Finnish Centre for Laboratory Animal Pathology/HiLIFE, University of Helsinki, FIN-00014 Helsinki, Finland)

  • Tim Garrels

    (Department of Computing, University of Turku, FI-20014 Turku, Finland)

  • Satu Pekkala

    (Faculty of Sport and Health Sciences, University of Jyväskylä, FI-40014 Jyväskylä, Finland
    Department of Clinical Microbiology, Turku University Hospital, FI-20521 Turku, Finland
    These authors contributed equally as the last authors to this work.)

  • Leo Lahti

    (Department of Computing, University of Turku, FI-20014 Turku, Finland
    These authors contributed equally as the last authors to this work.)

Abstract

We have shown that prebiotic xylo-oligosaccharides (XOS) increased beneficial gut microbiota (GM) and prevented high fat diet-induced hepatic steatosis, but the mechanisms associated with these effects are not clear. We studied whether XOS affects adipose tissue inflammation and insulin signaling, and whether the GM and fecal metabolome explain associated patterns. XOS was supplemented or not with high (HFD) or low (LFD) fat diet for 12 weeks in male Wistar rats ( n = 10/group). Previously analyzed GM and fecal metabolites were biclustered to reduce data dimensionality and identify interpretable groups of co-occurring genera and metabolites. Based on our findings, biclustering provides a useful algorithmic method for capturing such joint signatures. On the HFD, XOS-supplemented rats showed lower number of adipose tissue crown-like structures, increased phosphorylation of AKT in liver and adipose tissue as well as lower expression of hepatic miRNAs. XOS-supplemented rats had more fecal glycine and less hypoxanthine, isovalerate, branched chain amino acids and aromatic amino acids. Several bacterial genera were associated with the metabolic signatures. In conclusion, the beneficial effects of XOS on hepatic steatosis involved decreased adipose tissue inflammation and likely improved insulin signaling, which were further associated with fecal metabolites and GM.

Suggested Citation

  • Jukka Hintikka & Sanna Lensu & Elina Mäkinen & Sira Karvinen & Marjaana Honkanen & Jere Lindén & Tim Garrels & Satu Pekkala & Leo Lahti, 2021. "Xylo-Oligosaccharides in Prevention of Hepatic Steatosis and Adipose Tissue Inflammation: Associating Taxonomic and Metabolomic Patterns in Fecal Microbiomes with Biclustering," IJERPH, MDPI, vol. 18(8), pages 1-24, April.
    • Handle: RePEc:gam:jijerp:v:18:y:2021:i:8:p:4049-:d:534532
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    References listed on IDEAS

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    1. Jorge Henao-Mejia & Eran Elinav & Chengcheng Jin & Liming Hao & Wajahat Z. Mehal & Till Strowig & Christoph A. Thaiss & Andrew L. Kau & Stephanie C. Eisenbarth & Michael J. Jurczak & Joao-Paulo Campor, 2012. "Inflammasome-mediated dysbiosis regulates progression of NAFLD and obesity," Nature, Nature, vol. 482(7384), pages 179-185, February.
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