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SARS-CoV-2 Short-Time Infection Produces Relevant Cytopathic Effects in Vero E6 Cell Line

Author

Listed:
  • Luisa Zupin

    (Institute for Maternal and Child Health IRCCS Burlo Garofolo, 34137 Trieste, Italy)

  • Francesco Fontana

    (Division of Laboratory Medicine, University Hospital Giuliano Isontina (ASUGI), 34129 Trieste, Italy)

  • Rossella Gratton

    (Institute for Maternal and Child Health IRCCS Burlo Garofolo, 34137 Trieste, Italy)

  • Margherita Milani

    (Department of Medicine, Surgery and Health Sciences, University of Trieste, 34149 Trieste, Italy)

  • Libera Clemente

    (Division of Laboratory Medicine, University Hospital Giuliano Isontina (ASUGI), 34129 Trieste, Italy)

  • Lorella Pascolo

    (Institute for Maternal and Child Health IRCCS Burlo Garofolo, 34137 Trieste, Italy)

  • Maurizio Ruscio

    (Division of Laboratory Medicine, University Hospital Giuliano Isontina (ASUGI), 34129 Trieste, Italy)

  • Sergio Crovella

    (Department of Biological and Environmental Sciences, College of Arts and Sciences, University of Qatar, Doha 2713, Qatar)

Abstract

Severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) is mainly transmitted through respiratory droplets from positive subjects to susceptible hosts or by direct contact with an infected individual. Our study focuses on the in vitro minimal time of viral absorption as well as the minimal quantity of virus able to establish a persistent infection in Vero E6 cells. We observed that 1 min of in vitro virus exposure is sufficient to generate a cytopathic effect in cells after 7 days of infection, even at a multiplicity of infection (MOI) value of 0.01. Being aware that our findings have been obtained using an in vitro cellular model, we demonstrated that short-time exposures and low viral concentrations are able to cause infection, thus opening questions about the risk of SARS-CoV-2 transmissibility even following short contact times.

Suggested Citation

  • Luisa Zupin & Francesco Fontana & Rossella Gratton & Margherita Milani & Libera Clemente & Lorella Pascolo & Maurizio Ruscio & Sergio Crovella, 2021. "SARS-CoV-2 Short-Time Infection Produces Relevant Cytopathic Effects in Vero E6 Cell Line," IJERPH, MDPI, vol. 18(17), pages 1-7, August.
    • Handle: RePEc:gam:jijerp:v:18:y:2021:i:17:p:9020-:d:622917
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    Citations

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    Cited by:

    1. Sabrina Semeraro & Anastasia Serena Gaetano & Luisa Zupin & Carlo Poloni & Elvio Merlach & Enrico Greco & Sabina Licen & Francesco Fontana & Silvana Leo & Alessandro Miani & Francesco Broccolo & Pierl, 2022. "Operative Protocol for Testing the Efficacy of Nasal Filters in Preventing Airborne Transmission of SARS-CoV-2," IJERPH, MDPI, vol. 19(21), pages 1-12, October.
    2. Luisa Zupin & Sabina Licen & Margherita Milani & Libera Clemente & Lorenzo Martello & Sabrina Semeraro & Francesco Fontana & Maurizio Ruscio & Alessandro Miani & Sergio Crovella & Pierluigi Barbieri, 2021. "Evaluation of Residual Infectivity after SARS-CoV-2 Aerosol Transmission in a Controlled Laboratory Setting," IJERPH, MDPI, vol. 18(21), pages 1-14, October.

    More about this item

    Keywords

    SARS-CoV-2; infection; Vero E6 cells;
    All these keywords.

    JEL classification:

    • E6 - Macroeconomics and Monetary Economics - - Macroeconomic Policy, Macroeconomic Aspects of Public Finance, and General Outlook

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