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The Acute Effects of Exercise and Temperature on Regional mtDNA

Author

Listed:
  • Mark L. McGlynn

    (School of Health and Kinesiology, University of Nebraska at Omaha, Omaha, NE 68182, USA)

  • Halee Schnitzler

    (School of Health and Kinesiology, University of Nebraska at Omaha, Omaha, NE 68182, USA)

  • Robert Shute

    (School of Health and Kinesiology, University of Nebraska at Omaha, Omaha, NE 68182, USA)

  • Brent Ruby

    (School of Integrative Physiology and Athletic Training, University of Montana, Missoula, MT 59812, USA)

  • Dustin Slivka

    (School of Health and Kinesiology, University of Nebraska at Omaha, Omaha, NE 68182, USA)

Abstract

A reduced mitochondrial DNA (mtDNA) copy number, the ratio of mitochondrial DNA to genomic DNA (mtDNA:gDNA), has been linked with dysfunctional mitochondria. Exercise can acutely induce mtDNA damage manifested as a reduced copy number. However, the influence of a paired (exercise and temperature) intervention on regional mtDNA (MINor Arc and MAJor Arc) are unknown. Thus, the purpose of this study was to determine the acute effects of exercise in cold (7 °C), room temperature (20 °C), and hot (33 °C) ambient temperatures, on regional mitochondrial copy number (MINcn and MAJcn). Thirty-four participants (24.4 ± 5.1 yrs, 87.1 ± 22.1 kg, 22.3 ± 8.5 %BF, and 3.20 ± 0.59 L·min −1 VO 2 peak) cycled for 1 h (261.1 ± 22.1 W) in either 7 °C, 20 °C, or 33 °C ambient conditions. Muscle biopsy samples were collected from the vastus lateralis to determine mtDNA regional copy numbers via RT-qPCR. mtDNA is sensitive to the stressors of exercise post-exercise (MIN fold change, −1.50 ± 0.11; MAJ fold change, −1.70 ± 0.12) and 4-h post-exercise (MIN fold change, −0.82 ± 0.13; MAJ fold change, −1.54 ± 0.11). The MAJ Arc seems to be more sensitive to heat, showing a temperature-trend ( p = 0.056) for a reduced regional copy number ratio after exercise in the heat (fold change −2.81 ± 0.11; p = 0.019). These results expand upon our current knowledge of the influence of temperature and exercise on the acute remodeling of regional mtDNA.

Suggested Citation

  • Mark L. McGlynn & Halee Schnitzler & Robert Shute & Brent Ruby & Dustin Slivka, 2021. "The Acute Effects of Exercise and Temperature on Regional mtDNA," IJERPH, MDPI, vol. 18(12), pages 1-12, June.
    • Handle: RePEc:gam:jijerp:v:18:y:2021:i:12:p:6382-:d:574149
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    Cited by:

    1. Monica Kwon & Larry Robins & Mark L. McGlynn & Christopher Collins & Elizabeth J. Pekas & Song-Young Park & Dustin Slivka, 2022. "No Mitochondrial Related Transcriptional Changes in Human Skeletal Muscle after Local Heat Application," IJERPH, MDPI, vol. 19(24), pages 1-10, December.

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