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Comet Test in Saliva Leukocytes of Pre-School Children Exposed to Air Pollution in North Italy: The Respira Study

Author

Listed:
  • Claudia Zani

    (Department of Medical and Surgical Specialties, Radiological Sciences and Public Health, University of Brescia, 11 Viale Europa, 25123 Brescia, Italy)

  • Elisabetta Ceretti

    (Department of Medical and Surgical Specialties, Radiological Sciences and Public Health, University of Brescia, 11 Viale Europa, 25123 Brescia, Italy)

  • Ilaria Zerbini

    (Department of Medical and Surgical Specialties, Radiological Sciences and Public Health, University of Brescia, 11 Viale Europa, 25123 Brescia, Italy)

  • Gaia Claudia Viviana Viola

    (Department of Medical and Surgical Specialties, Radiological Sciences and Public Health, University of Brescia, 11 Viale Europa, 25123 Brescia, Italy)

  • Francesco Donato

    (Department of Medical and Surgical Specialties, Radiological Sciences and Public Health, University of Brescia, 11 Viale Europa, 25123 Brescia, Italy)

  • Umberto Gelatti

    (Department of Medical and Surgical Specialties, Radiological Sciences and Public Health, University of Brescia, 11 Viale Europa, 25123 Brescia, Italy)

  • Donatella Feretti

    (Department of Medical and Surgical Specialties, Radiological Sciences and Public Health, University of Brescia, 11 Viale Europa, 25123 Brescia, Italy)

Abstract

Air pollution is a well-known problem for human health, especially for children living in highly polluted urban areas. This study aimed to assess the relationship between airborne pollutants concentration and biomarkers of DNA damage in the buccal mucosa cells of pre-school children. DNA damage was investigated with comet test in saliva leukocytes taken from sputum of 3- to 6-year-old children living in Brescia, Northern Italy, collected during two consecutive winter seasons (2012–2013). The daily levels of PM10, PM2.5, NO 2 , CO, SO 2 , benzene and O 3 in urban air were collected for the whole period. A questionnaire filled in by the children’s parents was used to evaluate indoor and outdoor exposure. DNA damage in saliva leukocytes was evaluated in 152 children and the means of tail intensity and visual score as DNA damage were 6.2 ± 4.3 and 182.1 ± 30.9, respectively. No demographic and indoor or outdoor exposure variable was associated with the two measures of DNA damage. No significant association between air pollution and DNA damage in children’s buccal leukocytes was found. In this study, the comet assay does not appear to be a valuable biomarker to detect DNA damage in children exposed to high levels of air pollutants, such as PM10, PM2.5 and NO 2 .

Suggested Citation

  • Claudia Zani & Elisabetta Ceretti & Ilaria Zerbini & Gaia Claudia Viviana Viola & Francesco Donato & Umberto Gelatti & Donatella Feretti, 2020. "Comet Test in Saliva Leukocytes of Pre-School Children Exposed to Air Pollution in North Italy: The Respira Study," IJERPH, MDPI, vol. 17(9), pages 1-11, May.
    • Handle: RePEc:gam:jijerp:v:17:y:2020:i:9:p:3276-:d:355219
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    Citations

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    Cited by:

    1. Diana Marín & Luz Yaneth Orozco & Diana María Narváez & Isabel Cristina Ortiz-Trujillo & Francisco José Molina & Carlos Daniel Ramos & Laura Rodriguez-Villamizar & Shrikant I Bangdiwala & Olga Morales, 2023. "Characterization of the external exposome and its contribution to the clinical respiratory and early biological effects in children: The PROMESA cohort study protocol," PLOS ONE, Public Library of Science, vol. 18(1), pages 1-15, January.
    2. Claudia Zani & Francesco Donato & Elisabetta Ceretti & Roberta Pedrazzani & Ilaria Zerbini & Umberto Gelatti & Donatella Feretti, 2021. "Genotoxic Activity of Particulate Matter and In Vivo Tests in Children Exposed to Air Pollution," IJERPH, MDPI, vol. 18(10), pages 1-13, May.
    3. Carla Russo & Mattia Acito & Cristina Fatigoni & Milena Villarini & Massimo Moretti, 2020. "B-Comet Assay (Comet Assay on Buccal Cells) for the Evaluation of Primary DNA Damage in Human Biomonitoring Studies," IJERPH, MDPI, vol. 17(24), pages 1-14, December.

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