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Analysis of Human Papillomavirus (HPV) 16 Variants Associated with Cervical Infection in Italian Women

Author

Listed:
  • Marianna Martinelli

    (Department of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, Italy)

  • Chiara Villa

    (Department of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, Italy)

  • Giovanni Sotgiu

    (Department of Medical, Surgical and Experimental Sciences, University of Sassari, 07100 Sassari, Italy)

  • Narcisa Muresu

    (Department of Medical, Surgical and Experimental Sciences, University of Sassari, 07100 Sassari, Italy)

  • Federica Perdoni

    (Department of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, Italy)

  • Rosario Musumeci

    (Department of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, Italy)

  • Romina Combi

    (Department of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, Italy)

  • Antonio Cossu

    (Department of Medical, Surgical and Experimental Sciences, University of Sassari, 07100 Sassari, Italy)

  • Andrea Piana

    (Department of Medical, Surgical and Experimental Sciences, University of Sassari, 07100 Sassari, Italy)

  • Clementina Cocuzza

    (Department of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, Italy)

Abstract

This study aims to evaluate HPV16 variants distribution in a population of Italian women living in two different regions (Lombardy and Sardinia) by sequence analyses of HPV16-positive cervical samples, in order to reconstruct the phylogenetic relationship among variants to identify the currently circulating lineages. Analyses were conducted starting from DNA isolated from 67 HPV16-positive cervical samples collected from two different Italian centres (31 from Lombardy and 36 from Sardinia) of women with normal and abnormal cervical cytology. The entire long control region (LCR) and 300 nt of the E6 gene was sequenced to identify intra-type variants. Sequence comparison and phylogenetic analysis were made using a distance-based neighbour joining method (NJ) and Kimura two-parameter model. Data obtained reported that Italian sequences mainly belonged to the European lineage, in particular sublineage A2. Only five sequences clustered in non-European branches: two in North American lineage (sublineage D1), two in African-1 (sublineage B1) and one in African-2. A new 27 nucleotide duplication in the central segment of the LCR region was found in a sequence obtained from a sample isolated in Sardinia. A predominance of European variants was detected, with some degree of variability among the studied HPV16 strains. This study contributes to the implementation of data regarding the molecular epidemiology of HPV16 variants.

Suggested Citation

  • Marianna Martinelli & Chiara Villa & Giovanni Sotgiu & Narcisa Muresu & Federica Perdoni & Rosario Musumeci & Romina Combi & Antonio Cossu & Andrea Piana & Clementina Cocuzza, 2020. "Analysis of Human Papillomavirus (HPV) 16 Variants Associated with Cervical Infection in Italian Women," IJERPH, MDPI, vol. 17(1), pages 1-10, January.
    • Handle: RePEc:gam:jijerp:v:17:y:2020:i:1:p:306-:d:304227
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    References listed on IDEAS

    as
    1. Marianna Martinelli & Rosario Musumeci & Alberto Rizzo & Narcisa Muresu & Andrea Piana & Giovanni Sotgiu & Fabio Landoni & Clementina Cocuzza, 2019. "Prevalence of Chlamydia trachomatis Infection, Serovar Distribution and Co-Infections with Seven High-Risk HPV Types among Italian Women with a Recent History of Abnormal Cervical Cytology," IJERPH, MDPI, vol. 16(18), pages 1-10, September.
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    1. Marianna Martinelli & Rosario Musumeci & Illari Sechi & Giovanni Sotgiu & Andrea Piana & Federica Perdoni & Federica Sina & Robert Fruscio & Fabio Landoni & Clementina E. Cocuzza, 2019. "Prevalence of Human Papillomavirus (HPV) and Other Sexually Transmitted Infections (STIs) among Italian Women Referred for a Colposcopy," IJERPH, MDPI, vol. 16(24), pages 1-11, December.

    More about this item

    Keywords

    HPV genotypes; HPV variants; LCR mutations; HPV16;
    All these keywords.

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