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Design and Validation with Influenza A Virus of an Aerosol Transmission Chamber for Ferrets

Author

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  • Nathalie Turgeon

    (Centre de Recherche de l’Institut Universitaire de Cardiologie et de Pneumologie de Québec, 2725 Chemin Ste-Foy, Québec City, QC G1V 4G5, Canada
    Département de Biochimie, de Microbiologie et de Bio-Informatique, Faculté des sciences et de génie, Université Laval, Québec City, QC G1V 0A6, Canada)

  • Marie-Ève Hamelin

    (Centre de Recherche du Centre Hospitalier Universitaire de Québec, CHUL, 2705 boulevard Laurier, Québec City, QC G1V 4G2, Canada)

  • Daniel Verreault

    (Research and Development Institute for the Agri-Environment, 2700 rue Einstein, Québec City, QC G1P 3W8, Canada)

  • Ariane Lévesque

    (Research and Development Institute for the Agri-Environment, 2700 rue Einstein, Québec City, QC G1P 3W8, Canada)

  • Chantal Rhéaume

    (Centre de Recherche du Centre Hospitalier Universitaire de Québec, CHUL, 2705 boulevard Laurier, Québec City, QC G1V 4G2, Canada)

  • Julie Carbonneau

    (Centre de Recherche du Centre Hospitalier Universitaire de Québec, CHUL, 2705 boulevard Laurier, Québec City, QC G1V 4G2, Canada)

  • Liva Checkmahomed

    (Centre de Recherche du Centre Hospitalier Universitaire de Québec, CHUL, 2705 boulevard Laurier, Québec City, QC G1V 4G2, Canada)

  • Matthieu Girard

    (Research and Development Institute for the Agri-Environment, 2700 rue Einstein, Québec City, QC G1P 3W8, Canada)

  • Guy Boivin

    (Centre de Recherche du Centre Hospitalier Universitaire de Québec, CHUL, 2705 boulevard Laurier, Québec City, QC G1V 4G2, Canada)

  • Caroline Duchaine

    (Centre de Recherche de l’Institut Universitaire de Cardiologie et de Pneumologie de Québec, 2725 Chemin Ste-Foy, Québec City, QC G1V 4G5, Canada
    Département de Biochimie, de Microbiologie et de Bio-Informatique, Faculté des sciences et de génie, Université Laval, Québec City, QC G1V 0A6, Canada)

Abstract

Background : The importance of aerosols in the spread of viruses like influenza is still a subject of debate. Indeed, most viruses can also be transmitted through direct contact and droplets. Therefore, the importance of the airborne route in a clinical context is difficult to determine. The aim of this study was to design a chamber system to study the airborne transmission of viruses between ferrets. Methods : A system composed of three chambers connected in series, each one housing one ferret and preventing direct contact, was designed. The chambers were designed to house the ferrets for several days and to study the transmission of viruses from an infected (index) ferret to two naïve ferrets via aerosols and droplets or aerosols only. A particle separator was designed that can be used to modulate the size of the particles traveling between the chambers. The chamber system was validated using standard dust as well as with ferrets infected with influenza A virus. Conclusions : The 50% efficiency cut-off of the separator could be modulated between a 5-µm and an 8-µm aerodynamic diameter. In the described setup, influenza A virus was transmitted through the aerosol route in two out of three experiments, and through aerosols and droplets in all three experiments.

Suggested Citation

  • Nathalie Turgeon & Marie-Ève Hamelin & Daniel Verreault & Ariane Lévesque & Chantal Rhéaume & Julie Carbonneau & Liva Checkmahomed & Matthieu Girard & Guy Boivin & Caroline Duchaine, 2019. "Design and Validation with Influenza A Virus of an Aerosol Transmission Chamber for Ferrets," IJERPH, MDPI, vol. 16(4), pages 1-10, February.
    • Handle: RePEc:gam:jijerp:v:16:y:2019:i:4:p:609-:d:207337
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    References listed on IDEAS

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    1. Mathilde Richard & Eefje J. A. Schrauwen & Miranda de Graaf & Theo M. Bestebroer & Monique I. J. Spronken & Sander van Boheemen & Dennis de Meulder & Pascal Lexmond & Martin Linster & Sander Herfst & , 2013. "Limited airborne transmission of H7N9 influenza A virus between ferrets," Nature, Nature, vol. 501(7468), pages 560-563, September.
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    Cited by:

    1. Nan Zhang & Boni Su & Pak-To Chan & Te Miao & Peihua Wang & Yuguo Li, 2020. "Infection Spread and High-Resolution Detection of Close Contact Behaviors," IJERPH, MDPI, vol. 17(4), pages 1-18, February.

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