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Measurements of Oxidative Potential of Particulate Matter at Belgrade Tunnel; Comparison of BPEAnit, DTT and DCFH Assays

Author

Listed:
  • Maja V. Jovanovic

    (Vinca Institute of Nuclear Sciences, University of Belgrade, P.O. Box 522, 11001 Belgrade, Serbia)

  • Jasmina Z. Savic

    (Vinca Institute of Nuclear Sciences, University of Belgrade, P.O. Box 522, 11001 Belgrade, Serbia)

  • Farhad Salimi

    (University Centre for Rural Health–North Coast, School of Public Health, University of Sydney, Sydney, NSW 2006, Australia
    Centre for Air Quality & Health Research and Evaluation (CAR), An NHMRC Centre of Research Excellence, Glebe, NSW 2037, Australia)

  • Svetlana Stevanovic

    (School of Engineering, Deakin University, Melbourne, VIC 3216, Australia)

  • Reece A. Brown

    (ILAQH (International Laboratory of Air Quality and Health), Queensland University of Technology, 2 George St., Brisbane, QLD 4000, Australia)

  • Milena Jovasevic-Stojanovic

    (Vinca Institute of Nuclear Sciences, University of Belgrade, P.O. Box 522, 11001 Belgrade, Serbia)

  • Dragan Manojlovic

    (Faculty of Chemistry, University of Belgrade, Studentski trg 12–16, 11000 Belgrade, Serbia
    South Ural State University, Lenin prospect 76, 454080 Chelyabinsk, Russia)

  • Alena Bartonova

    (NILU–Norwegian Institute for Air Research, P.O. Box 100, 2027 Kjeller, Norway)

  • Steven Bottle

    (School of Chemistry, Physics and Mechanical Engineering, Queensland University of Technology (QUT), Brisbane, QLD 4000, Australia)

  • Zoran D. Ristovski

    (ILAQH (International Laboratory of Air Quality and Health), Queensland University of Technology, 2 George St., Brisbane, QLD 4000, Australia
    School of Chemistry, Physics and Mechanical Engineering, Queensland University of Technology (QUT), Brisbane, QLD 4000, Australia)

Abstract

To estimate the oxidative potential (OP) of particulate matter (PM), two commonly used cell-free, molecular probes were applied: dithiothreitol (DTT) and dichloro-dihydro-fluorescein diacetate (DCFH-DA), and their performance was compared with 9,10-bis (phenylethynyl) anthracene-nitroxide (BPEAnit). To the best of our knowledge, this is the first study in which the performance of the DTT and DCFH has been compared with the BPEAnit probe. The average concentrations of PM, organic carbon (OC) and elemental carbon (EC) for fine (PM 2.5 ) and coarse (PM 10 ) particles were determined. The results were 44.8 ± 13.7, 9.8 ± 5.1 and 9.3 ± 4.8 µg·m −3 for PM 2.5 and 75.5 ± 25.1, 16.3 ± 8.7 and 11.8 ± 5.3 µg·m −3 for PM 10 , respectively, for PM, OC and EC. The water-soluble organic carbon (WSOC) fraction accounted for 42 ± 14% and 28 ± 9% of organic carbon in PM 2.5 and PM 10 , respectively. The average volume normalized OP values for the three assays depended on both the sampling periods and the PM fractions. The OP BPEAnit had its peak at 2 p.m.; in the afternoon, it was three times higher compared to the morning and late afternoon values. The DCFH and BPEAnit results were correlated ( r = 0.64), while there was no good agreement between the BPEAnit and the DTT ( r = 0.14). The total organic content of PM does not necessarily represent oxidative capacity and it shows varying correlation with the OP. With respect to the two PM fractions studied, the OP was mostly associated with smaller particles.

Suggested Citation

  • Maja V. Jovanovic & Jasmina Z. Savic & Farhad Salimi & Svetlana Stevanovic & Reece A. Brown & Milena Jovasevic-Stojanovic & Dragan Manojlovic & Alena Bartonova & Steven Bottle & Zoran D. Ristovski, 2019. "Measurements of Oxidative Potential of Particulate Matter at Belgrade Tunnel; Comparison of BPEAnit, DTT and DCFH Assays," IJERPH, MDPI, vol. 16(24), pages 1-17, December.
    • Handle: RePEc:gam:jijerp:v:16:y:2019:i:24:p:4906-:d:294334
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