IDEAS home Printed from https://ideas.repec.org/a/gam/jijerp/v15y2018i8p1695-d162755.html
   My bibliography  Save this article

Use of MALDI-TOF MS to Discriminate between Biofilm-Producer and Non-Producer Strains of Staphylococcus epidermidis

Author

Listed:
  • Pina Caputo

    (Department of Experimental Medicine, Section of Microbiology and Clinical Microbiology University of Campania “Luigi Vanvitelli”, 80138 Naples, Italy)

  • Maria Chiara Di Martino

    (Department of Experimental Medicine, Section of Microbiology and Clinical Microbiology University of Campania “Luigi Vanvitelli”, 80138 Naples, Italy)

  • Brunella Perfetto

    (Department of Experimental Medicine, Section of Microbiology and Clinical Microbiology University of Campania “Luigi Vanvitelli”, 80138 Naples, Italy)

  • Francesco Iovino

    (Division of General Surgery, Department of Cardiothoracic and Respiratory Science, University of Campania “Luigi Vanvitelli”, 80138 Naples, Italy)

  • Giovanna Donnarumma

    (Department of Experimental Medicine, Section of Microbiology and Clinical Microbiology University of Campania “Luigi Vanvitelli”, 80138 Naples, Italy)

Abstract

For the management of Staphylococci coagulase-negative infection, often related to biofilm formation, rapid and accurate identification is necessary in choosing a correct antibiotic therapy. Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) is becoming increasingly important for bacterial identification over traditional methods. Our aim was to validate the use of MALDI to discriminate Staphylococcus epidermidis biofilm-producing strains. Clinical strains coming from suture wires were identified and their protein profiles were compared to that obtained from two ATCC reference strains (biofilm producer and non-producer). MALDI identified the eighteen isolates as S. epidermidis , combining sixteen profiles with the biofilm producer and two with the non-producer, confirming the results of crystal violet assay. Our data highlight that MALDI can be considered a good tool to discriminate between biofilm-producer and non-producer strains of S. epidermidis , thus helping to establish an effective antibiotic therapy.

Suggested Citation

  • Pina Caputo & Maria Chiara Di Martino & Brunella Perfetto & Francesco Iovino & Giovanna Donnarumma, 2018. "Use of MALDI-TOF MS to Discriminate between Biofilm-Producer and Non-Producer Strains of Staphylococcus epidermidis," IJERPH, MDPI, vol. 15(8), pages 1-11, August.
  • Handle: RePEc:gam:jijerp:v:15:y:2018:i:8:p:1695-:d:162755
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/1660-4601/15/8/1695/pdf
    Download Restriction: no

    File URL: https://www.mdpi.com/1660-4601/15/8/1695/
    Download Restriction: no
    ---><---

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jijerp:v:15:y:2018:i:8:p:1695-:d:162755. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.