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Impact of Vegetative Treatment Systems on Multiple Measures of Antibiotic Resistance in Agricultural Wastewater

Author

Listed:
  • Lisa M. Durso

    (United States Department of Agriculture (USDA), Agricultural Research Service (ARS), Agroecosystem Management Research Unit, 251 Filley Hall, UNL East Campus Lincoln, Lincoln, NE 68583, USA)

  • Daniel N. Miller

    (United States Department of Agriculture (USDA), Agricultural Research Service (ARS), Agroecosystem Management Research Unit, 251 Filley Hall, UNL East Campus Lincoln, Lincoln, NE 68583, USA)

  • Christopher G. Henry

    (Rice Research and Extension Center, University of Arkansas, Stuttgart, AR 72160, USA
    Previously Biological Systems Engineering, University of Nebraska-Lincoln, Lincoln, NE 68583, USA)

Abstract

Wastewater is an important vector of antibiotic resistant bacteria and antibiotic resistance genes (ARB/G). While there is broad agreement that ARB/G from agricultural (ag) wastewaters can be transported through the environment and may contribute to untreatable infectious disease in humans and animals, there remain large knowledge gaps surrounding applied details on the types and amounts of ARB/G associated with different agricultural wastewater treatment options and different ag production systems. This study evaluates a vegetative treatment system (VTS) built to treat the wastewater from a beef cattle feedlot. Samples were collected for three years, and plated on multiple media types to enumerate tetracycline and cefotaxime-resistant bacteria. Enterobacteriaceae isolates ( n = 822) were characterized for carriage of tetracycline resistance genes, and E. coli isolates ( n = 673) were phenotyped to determine multi-drug resistance (MDR) profiles. Tetracycline resistance in feedlot runoff wastewater was 2-to-3 orders of magnitude higher compared to rainfall runoff from the VTS fields, indicating efficacy of the VTA for reducing ARB over time following wastewater application. Clear differences in MDR profiles were observed based on the specific media on which a sample was plated. This result highlights the importance of method, especially in the context of isolate-based surveillance and monitoring of ARB in agricultural wastewaters.

Suggested Citation

  • Lisa M. Durso & Daniel N. Miller & Christopher G. Henry, 2018. "Impact of Vegetative Treatment Systems on Multiple Measures of Antibiotic Resistance in Agricultural Wastewater," IJERPH, MDPI, vol. 15(7), pages 1-13, June.
    • Handle: RePEc:gam:jijerp:v:15:y:2018:i:7:p:1295-:d:153560
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    Cited by:

    1. Alexandra Bastaraud & Philippe Cecchi & Pascal Handschumacher & Mathias Altmann & Ronan Jambou, 2020. "Urbanization and Waterborne Pathogen Emergence in Low-Income Countries: Where and How to Conduct Surveys?," IJERPH, MDPI, vol. 17(2), pages 1-19, January.

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