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Synthetic Receptors Induce Anti Angiogenic and Stress Signaling on Human First Trimester Cytotrophoblast Cells

Author

Listed:
  • Ahmed F. Pantho

    (Department of Biochemistry, University of Texas at Austin, Austin, TX 78712, USA)

  • Mason Price

    (Department of Biology, Angelo State University, San Angelo, TX 76904, USA)

  • AHM Zuberi Ashraf

    (Department of Obstetrics & Gynecology, Baylor Scott & White Health, Temple/Texas A&M Health Science Center College of Medicine, Temple, TX 76508, USA)

  • Umaima Wajid

    (Department of Obstetrics & Gynecology, Baylor Scott & White Health, Temple/Texas A&M Health Science Center College of Medicine, Temple, TX 76508, USA)

  • Maryam Emami Khansari

    (Department of Chemistry & Biochemistry, Jackson State University, Jackson, MS 39217, USA)

  • Afsana Jahan

    (Department of Chemistry & Biochemistry, Jackson State University, Jackson, MS 39217, USA)

  • Syeda H. Afroze

    (Medical Physiology, Texas A&M Health Science Center College of Medicine, Temple, TX 76504, USA)

  • Md Mhahabubur Rhaman

    (Department of Chemistry & Biochemistry, Jackson State University, Jackson, MS 39217, USA)

  • Corey R. Johnson

    (Department of Chemistry & Biochemistry, Jackson State University, Jackson, MS 39217, USA)

  • Thomas J. Kuehl

    (Department of Obstetrics & Gynecology, Baylor Scott & White Health, Temple/Texas A&M Health Science Center College of Medicine, Temple, TX 76508, USA
    Department of Pediatrics, Baylor Scott & White Health, Temple/Texas A&M Health Science Center College of Medicine, Temple, TX 76508, USA)

  • Md. Alamgir Hossain

    (Department of Chemistry & Biochemistry, Jackson State University, Jackson, MS 39217, USA)

  • Mohammad Nasir Uddin

    (Department of Obstetrics & Gynecology, Baylor Scott & White Health, Temple/Texas A&M Health Science Center College of Medicine, Temple, TX 76508, USA
    Department of Pediatrics, Baylor Scott & White Health, Temple/Texas A&M Health Science Center College of Medicine, Temple, TX 76508, USA
    Internal Medicine, Baylor Scott & White Health, Temple/Texas A&M Health Science Center College of Medicine, Temple, TX 76508, USA)

Abstract

The cytotrophoblast (CTB) cells of the human placenta have membrane receptors that bind certain cardiotonic steroids (CTS) found in blood plasma. One of these, marinobufagenin, is a key factor in the etiology of preeclampsia. Herein, we used synthetic receptors (SR) to study their effectiveness on the angiogenic profile of human first trimester CTB cells. The humanextravillous CTB cells (Sw.71) used in this study were derived from first trimester chorionic villus tissue. Culture media of CTB cells treated with ≥1 nM SR level revealed sFlt-1 (Soluble fms-like tyrosine kinase-1) was significantly increased while VEGF (vascular endothelial growth factor) was significantly decreased in the culture media (* p < 0.05 for each) The AT 2 receptor (Angiotensin II receptor type 2) expression was significantly upregulated in ≥1 nM SR-treated CTB cells as compared to basal; however, the AT 1 (Angiotensin II receptor, type 1) and VEGFR-1 (vascular endothelial growth factor receptor 1) receptor expression was significantly downregulated (* p < 0.05 for each). Our results show that the anti-proliferative and anti-angiogenic effects of SR on CTB cells are similar to the effects of CTS. The observed anti angiogenic activity of SR on CTB cells demonstrates that the functionalized-urea/thiourea molecules may be useful as potent inhibitors to prevent CTS-induced impairment of CTB cells.

Suggested Citation

  • Ahmed F. Pantho & Mason Price & AHM Zuberi Ashraf & Umaima Wajid & Maryam Emami Khansari & Afsana Jahan & Syeda H. Afroze & Md Mhahabubur Rhaman & Corey R. Johnson & Thomas J. Kuehl & Md. Alamgir Hoss, 2017. "Synthetic Receptors Induce Anti Angiogenic and Stress Signaling on Human First Trimester Cytotrophoblast Cells," IJERPH, MDPI, vol. 14(5), pages 1-15, May.
    • Handle: RePEc:gam:jijerp:v:14:y:2017:i:5:p:517-:d:98274
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    References listed on IDEAS

    as
    1. Darijana Horvat & Maryam Emami Khansari & Avijit Pramanik & Madhava R. Beeram & Thomas J. Kuehl & Md. Alamgir Hossain & Mohammad Nasir Uddin, 2014. "A Synthetic Thiourea-Based Tripodal Receptor that Impairs the Function of Human First Trimester Cytotrophoblast Cells," IJERPH, MDPI, vol. 11(7), pages 1-14, July.
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