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Effects of Ambient Fine Particles PM 2.5 on Human HaCaT Cells

Author

Listed:
  • Qiao Li

    (Department of Dermatology, Huashan Hospital, Shanghai Medical School, Fudan University, Shanghai 200040, China
    These authors contributed equally to this study.)

  • Zhihua Kang

    (Department of Laboratory Medicine, Huashan Hospital, Shanghai Medical School, Fudan University, Shanghai 200040, China
    These authors contributed equally to this study.)

  • Shuo Jiang

    (Department of Environmental Health, School of Public Health and the Key Laboratory of Public Health Safety, Ministry of Education, Fudan University, Shanghai 200032, China
    These authors contributed equally to this study.)

  • Jinzhuo Zhao

    (Department of Environmental Health, School of Public Health and the Key Laboratory of Public Health Safety, Ministry of Education, Fudan University, Shanghai 200032, China)

  • Shuxian Yan

    (Department of Dermatology, Huashan Hospital, Shanghai Medical School, Fudan University, Shanghai 200040, China)

  • Feng Xu

    (Department of Dermatology, Huashan Hospital, Shanghai Medical School, Fudan University, Shanghai 200040, China)

  • Jinhua Xu

    (Department of Dermatology, Huashan Hospital, Shanghai Medical School, Fudan University, Shanghai 200040, China)

Abstract

The current study was conducted to observe the effects of fine particulate matter (PM 2.5 ) on human keratinocyte cell line (HaCaT) cells. The potential mechanism linking PM 2.5 and skin was explored. HaCaT cells were cultured and then accessed in plate with PM 2.5 . Cell viability was tested by Cell Counting Kit-8. The mRNA and protein expression of Filaggrin, Loricrin, Involucrin, and Repetin were analyzed. The levels of Granulocyte-macrophage Colony Stimulating Factor, Thymic Stromal Lymphopoietin, Tumor Necrosis Factor-α, Interleukin-1α, and Interleukin-8 were detected in the supernatant of the HaCaT cell with enzyme-linked immunosorbent assay kits. Cell viability decreased with the increase in PM 2.5 . Compared with the control group, the protein expression of Filaggrin, Repetin, Involucrin, and Loricrin showed different expression patterns in PM 2.5 treatment groups. The level of Tumor Necrosis Factor-α, Thymic Stromal Lymphopoietin, Interleukin-1α, and Interleukin-8 significantly increased in the cells treated with PM 2.5 . Ambient PM 2.5 may increase the risk of eczema and other skin diseases. The relative mechanism may be associated with the impairment of the skin barrier and the elevation of inflammatory responses.

Suggested Citation

  • Qiao Li & Zhihua Kang & Shuo Jiang & Jinzhuo Zhao & Shuxian Yan & Feng Xu & Jinhua Xu, 2017. "Effects of Ambient Fine Particles PM 2.5 on Human HaCaT Cells," IJERPH, MDPI, vol. 14(1), pages 1-10, January.
    • Handle: RePEc:gam:jijerp:v:14:y:2017:i:1:p:72-:d:87655
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    Cited by:

    1. Shirin Kahremany & Lukas Hofmann & Noy Eretz-Kdosha & Eldad Silberstein & Arie Gruzman & Guy Cohen, 2021. "SH-29 and SK-119 Attenuates Air-Pollution Induced Damage by Activating Nrf2 in HaCaT Cells," IJERPH, MDPI, vol. 18(23), pages 1-16, November.

    More about this item

    Keywords

    PM 2.5 ; HaCaT cell; skin damage;
    All these keywords.

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