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A Marine Bacterium, Bacillus sp. Isolated from the Sediment Samples of Algoa Bay in South Africa Produces a Polysaccharide-Bioflocculant

Author

Listed:
  • Ncedo Ntozonke

    (SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa
    Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa)

  • Kunle Okaiyeto

    (SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa
    Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa)

  • Arinze S. Okoli

    (GenØK-Centre for Biosafety, Forskningsparken i Breivika, Postboks 6418, 9294 Tromsø, Norway)

  • Ademola O. Olaniran

    (Department of Microbiology, School of Life Sciences, University of KwaZulu-Natal, Private Bag X54001, Durban 4000, South Africa)

  • Uchechukwu U. Nwodo

    (SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa
    Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa)

  • Anthony I. Okoh

    (SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa
    Applied and Environmental Microbiology Research Group (AEMREG), Department of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa)

Abstract

Bioflocculants mediate the removal of suspended particles from solution and the efficiency of flocculation is dependent on the characteristics of the flocculant. Apart from the merits of biodegradability and harmlessness, bioflocculants could be viable as industrially relevant flocculants as they are a renewable resource. Additionally, the shortcomings associated with the conventionally used flocculants such as aluminium salts and acrylamide polymers, which include dementia and cancer, highlight more the need to use bioflocculants as an alternative. Consequently, in this study a marine sediment bacterial isolate was screened for bioflocculant production. Basic local alignment search tools (BLAST) analysis of 16S ribosomal deoxyribonucleic acid (rDNA) sequence of the bacterial isolate showed 98% similarity to Bacillus thuringiensis MR-R1. The bacteria produced bioflocculant optimally with inoculum size (4% v/v ) (85%), glucose (85.65%) and mixed nitrogen source (urea, ammonium chloride and yeast extract) (75.9%) and the divalent cation (Ca 2+ ) (62.3%). Under optimal conditions, a maximum flocculating activity of over 85% was attained after 60 h of cultivation. The purified polysaccharide-bioflocculant flocculated optimally at alkaline pH 12 (81%), in the presence of Mn 2+ (73%) and Ca 2+ (72.8%). The high flocculation activity shown indicates that the bioflocculant may contend favourably as an alternative to the conventionally used flocculants in water treatment.

Suggested Citation

  • Ncedo Ntozonke & Kunle Okaiyeto & Arinze S. Okoli & Ademola O. Olaniran & Uchechukwu U. Nwodo & Anthony I. Okoh, 2017. "A Marine Bacterium, Bacillus sp. Isolated from the Sediment Samples of Algoa Bay in South Africa Produces a Polysaccharide-Bioflocculant," IJERPH, MDPI, vol. 14(10), pages 1-14, September.
    • Handle: RePEc:gam:jijerp:v:14:y:2017:i:10:p:1149-:d:113632
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    References listed on IDEAS

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    1. Kunle Okaiyeto & Uchechukwu U. Nwodo & Leonard V. Mabinya & Anthony I. Okoh, 2013. "Characterization of a Bioflocculant Produced by a Consortium of Halomonas sp. Okoh and Micrococcus sp. Leo," IJERPH, MDPI, vol. 10(10), pages 1-14, October.
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    Cited by:

    1. Tsolanku Sidney Maliehe & Albertus Kotze Basson & Nkosinathi Goodman Dlamini, 2019. "Removal of Pollutants in Mine Wastewater by a Non-Cytotoxic Polymeric Bioflocculant from Alcaligenes faecalis HCB2," IJERPH, MDPI, vol. 16(20), pages 1-17, October.

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