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Cytotoxic Evaluation of e-Liquid Aerosol using Different Lung-Derived Cell Models

Author

Listed:
  • Stefanie Scheffler

    (Cultex Laboratories GmbH, Feodor-Lynen-Street 21, 30625 Hannover, Germany)

  • Hauke Dieken

    (Cultex Laboratories GmbH, Feodor-Lynen-Street 21, 30625 Hannover, Germany)

  • Olaf Krischenowski

    (Cultex Laboratories GmbH, Feodor-Lynen-Street 21, 30625 Hannover, Germany)

  • Michaela Aufderheide

    (Cultex Laboratories GmbH, Feodor-Lynen-Street 21, 30625 Hannover, Germany)

Abstract

The in vitro toxicological evaluation of e-liquid aerosol is an important aspect of consumer protection, but the cell model is of great significance. Due to its water solubility, e-liquid aerosol is deposited in the conducting zone of the respiratory tract. Therefore, primary normal human bronchial epithelial (NHBE) cells are more suitable for e-liquid aerosol testing than the widely used alveolar cell line A549. Due to their prolonged lifespan, immortalized cell lines derived from primary NHBE cells, exhibiting a comparable in vitro differentiation, might be an alternative for acute toxicity testing. In our study, A549 cells freshly isolated NHBE cells and the immortalized cell line CL-1548 were exposed at the air-liquid interface to e-liquid aerosol and cigarette mainstream smoke in a CULTEX ® RFS compact module. The cell viability was analyzed 24 h post-exposure. In comparison with primary NHBE cells, the CL-1548 cell line showed lower sensitivity to e-liquid aerosol but significantly higher sensitivity compared to A549 cells. Therefore, the immortalized cell line CL-1548 is recommended as a tool for the routine testing of e-liquid aerosol and is preferable to A549 cells.

Suggested Citation

  • Stefanie Scheffler & Hauke Dieken & Olaf Krischenowski & Michaela Aufderheide, 2015. "Cytotoxic Evaluation of e-Liquid Aerosol using Different Lung-Derived Cell Models," IJERPH, MDPI, vol. 12(10), pages 1-9, October.
    • Handle: RePEc:gam:jijerp:v:12:y:2015:i:10:p:12466-12474:d:56763
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    References listed on IDEAS

    as
    1. Stefanie Scheffler & Hauke Dieken & Olaf Krischenowski & Christine Förster & Detlev Branscheid & Michaela Aufderheide, 2015. "Evaluation of E-Cigarette Liquid Vapor and Mainstream Cigarette Smoke after Direct Exposure of Primary Human Bronchial Epithelial Cells," IJERPH, MDPI, vol. 12(4), pages 1-11, April.
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    Cited by:

    1. Riccardo Polosa & Massimo Caruso & Francesca Guarino, 2016. "Comments on Scheffler et al . Cytotoxic Evaluation of E-Liquid Aerosol using Different Lung Derived Cell Models. Int. J. Environ. Res. Public Health , 2015, 12 , 12466-12474," IJERPH, MDPI, vol. 13(1), pages 1-3, January.
    2. Jefferson Fowles & Tracy Barreau & Nerissa Wu, 2020. "Cancer and Non-Cancer Risk Concerns from Metals in Electronic Cigarette Liquids and Aerosols," IJERPH, MDPI, vol. 17(6), pages 1-10, March.

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