Enhanced Methane Production in the Anaerobic Digestion of Swine Manure: Effects of Substrate-to-Inoculum Ratio and Magnetite-Mediated Direct Interspecies Electron Transfer

Improving the anaerobic digestion (AD) of swine manure is crucial for sustainable waste-to-energy systems, given its high organic load and process instability risks. This study examined the combined effects of substrate-to-inoculum ratio (SIR, 0.1–3.2) and magnetite-mediated direct interspecies electron transfer on biogas production, effluent quality, and microbial community dynamics. The highest methane yield (262 ± 10 mL CH 4 /g COD) was obtained at SIR 0.1, while efficiency declined at higher SIRs due to acid and ammonia accumulation. Magnetite supplementation significantly improved methane yield (up to a 54.1% increase at SIR 0.2) and reduced the lag phase, particularly under moderate SIRs. Effluent characterization revealed that low SIRs induced elevated soluble COD (SCOD) levels, attributed to microbial autolysis and extracellular polymeric substance release. Furthermore, magnetite addition mitigated SCOD accumulation and shifted molecular weight distributions toward higher fractions (>15 kDa), indicating enhanced microbial activity and structural polymer formation. Microbial analysis revealed that magnetite-enriched Syntrophobacterium and Methanothrix promoted syntrophic cooperation and acetoclastic methanogenesis. Diversity indices and PCoA further showed that both SIR and magnetite significantly shaped microbial structure and function. Overall, an optimal SIR range of 0.2–0.4 under magnetite addition provided a balanced strategy for enhancing methane recovery, effluent quality, and microbial stability in swine manure AD.