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Optical Evaluation of Effects of Energy Substrates on PHB Accumulation for Bioplastic Production

Author

Listed:
  • Alicja Staśczak

    (Department of Automatic Control and Robotics, Silesian University of Technology, Akademicka 16, 44-100 Gliwice, Poland)

  • Hanna Langer-Macioł

    (Department of Automatic Control and Robotics, Silesian University of Technology, Akademicka 16, 44-100 Gliwice, Poland)

  • Karolina Widzisz

    (Department of Automatic Control and Robotics, Silesian University of Technology, Akademicka 16, 44-100 Gliwice, Poland)

  • Wiktoria Śliwińska

    (Department of Automatic Control and Robotics, Silesian University of Technology, Akademicka 16, 44-100 Gliwice, Poland)

  • Kinga Lucińska

    (Department of Automatic Control and Robotics, Silesian University of Technology, Akademicka 16, 44-100 Gliwice, Poland)

  • Przemysław Wencel

    (Department of Automatic Control and Robotics, Silesian University of Technology, Akademicka 16, 44-100 Gliwice, Poland)

  • Barbara Strózik

    (Department of Automatic Control and Robotics, Silesian University of Technology, Akademicka 16, 44-100 Gliwice, Poland)

  • Mariusz Frąckiewicz

    (Department of Data Science and Engineering, Silesian University of Technology, Akademicka 16, 44-100 Gliwice, Poland)

  • Piotr Skupin

    (Department of Automatic Control and Robotics, Silesian University of Technology, Akademicka 16, 44-100 Gliwice, Poland)

  • Dariusz Choiński

    (Department of Automatic Control and Robotics, Silesian University of Technology, Akademicka 16, 44-100 Gliwice, Poland)

  • Sebastian Student

    (Biotechnology Center, Silesian University of Technology, Krzywoustego 8, 44-100 Gliwice, Poland)

Abstract

To date, hundreds of millions tons of plastics has been produced worldwide. Their production and disposal are associated with high pollution and carbon release into the atmosphere. A more environmentally friendly alternative is bioplastics, and the most popular is polyhydroxybutyrate (PHB) polymer. Large amounts of PHB can be obtained from activated sludge where used cooking oil or other industrial waste can be used as potential substrates. In this work, efficient bioplastic production strategies are studied, and the considered substrate is a mixture of oil and peptone. Pseudomonas fluorescens bacteria are used to accumulate PHB, and the cultivation of microorganisms is carried out in batch and continuous-flow bioreactors. Microscopic observations and laboratory essays are performed to confirm presence of PHB and other key parameters. The obtained results allow us to determine the optimal feeding strategy.

Suggested Citation

  • Alicja Staśczak & Hanna Langer-Macioł & Karolina Widzisz & Wiktoria Śliwińska & Kinga Lucińska & Przemysław Wencel & Barbara Strózik & Mariusz Frąckiewicz & Piotr Skupin & Dariusz Choiński & Sebastian, 2022. "Optical Evaluation of Effects of Energy Substrates on PHB Accumulation for Bioplastic Production," Energies, MDPI, vol. 15(22), pages 1-16, November.
    • Handle: RePEc:gam:jeners:v:15:y:2022:i:22:p:8390-:d:968357
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