Is Plasma Treatment of Commodity Lettuce Seeds Worth It? Economic Impacts and Yield Study in Indoor Vertical Farming Testing Non-Thermal Plasmas

Agricultural seeds are sold as commodities yet seed quality can be non-uniform. Despite the extensive literature showing that plasma treatments of seeds provides advantages for many crops, lettuce studies, particularly in indoor farming systems, are limited. This study provides a systematic investigation of the impacts of non-thermal plasma treatments with various feed gases (N 2 , O 2 , dry air, and wet air) on the germination and growth characteristics of four lettuce cultivars (Red Oakleaf (RO), Black Simpson (BS), Valley Heart Romaine (VHR), and Paris Romaine (PR)) under controlled cultivation conditions in an agrivoltaic agrotunnel. Although the germination time was not conclusively affected by the treatments, the results show a complex interaction between germination rate and yield across the different cultivars and plasma treatments. Except for PR seeds (77.8% vs. 65.8% control), wet air plasma treatments increased germination rates by 18.7–100% over controls for all other cultivars. In yield analysis, wet air treatment had the strongest effect, especially for VHR (51.7 vs. 42.5 g/pot). Treatments did not notably affect RO. For BS, N 2 treatment gave the highest increase (54.2 vs. 48.1 g/pot), while PR responded best to O 2 treatment (58.4 vs. 51.8 g/pot). The energy consumption of plasma treatments was negligible for all treatments, while labor costs for small batches of seeds accounted for the largest share of secondary operating costs (839, 622, and 659 h/year, respectively for BS, VHR, and PR). Despite additional expenses, including labor, O&M, and degradation costs, the reduced seed requirements from higher germination rates and higher yield increased net profit by 12.0% compared to untreated cultivation in the most impacted (Valley Heart Romaine) lettuce. There is an opportunity for further cost optimization of the non-thermal plasma treatment for each type of lettuce seed.