Food Waste to Biogas: Continuous Operation of a Low-Cost Laboratory-Scale Anaerobic Digestion System Under Real-World Operating Constraints

This study evaluated low-cost food waste anaerobic digestion (FWAD) designed for African urban informal settlements, where electricity and process control are limited. Eight small-scale reactors were operated under varying mixing, pH control, and temperature conditions to assess the feasibility of stable operation with minimal input. Results showed no significant difference in methane yield between continuously mixed and minimally mixed (48-hourly) systems, nor between reactors with continuous pH dosing and those adjusted every 48 h (ANOVA p > 0.05 for all comparisons). The highest mean methane yield, 0.267 L CH 4 g VS −1 , was achieved by the minimally mixed reactor with 48-hourly pH control at 30 °C, while the controlled reactor at 37 °C produced a comparable 0.247 L CH 4 g VS −1 . Total methane production was similar at both temperatures, although gas generation was faster during the first 24 h at 37 °C. Compared to gas recovery achieved by extended batch operation following semi-continuous feeding, 58–73% of total methane was produced within the 48-h cycle, suggesting conversion could increase by 30–40% with extended liquid retention. Microbial analyses showed compositional differences but consistent performance, indicating functional redundancy within the microbial consortia. These results confirm the capacity of FWAD for stable, efficient biogas production without continuous energy input.