Analysis of Caffeine Content in Energy Drink and Its Impact on Students Health (by using UV-Visible Spectroscopy, HPTLC and Microscopy)

This study intends to examine the amount of caffeine in energy drinks, specifically sting, predator, Orello hydra , monster and evaluate any possible negative impacts on students who consume caffeinated energy drinks, such as a variety of health problem. The use of caffeinated energy drinks disrupts students sleep cycle and shortens their sleep length. Furthermore, studies have shown that children that ingest caffeinated have much greater blood sugar levels. This rise in the body’s toxicity may have negative effects. There is a connection between student deaths and the high caffeine level of caffeinated energy drinks. The beverages high sugar and caffeine content is the main cause of this. The study report also looks into several analytical techniques for figuring out how much caffeine is present in various body tissues There is also discussion about the negative effects of caffeine on the human body , particularly for those who drink it regularly. Caffeine addicts have been shown to have toxic side effects, such as elevated heart rate , restlessness, and anxiety. The study examines a number of analytical methods, such as UV-visible spectroscopy, TLC, and light microscope, to quantify the amount of caffeine. The techniques to effectively ascertain the caffeine content of an energy drink are provided in a step-by-step order. Overall, this study emphasizes the possible risks associated with ingesting energy drinks strong in caffeine, such as sting, especially for students. It is possible to limit caffeine consumption and lower health risks by being aware of the negative effects and putting good analytical procedures.