IDEAS home Printed from https://ideas.repec.org/a/epw/ejeng0/v4y2019i6id61384.html

Design of Construct Carrying GmDREB6 to Enhance Soybean Gene Expression Related to Abiotic Stress Response

Author

Listed:
  • Lan Thi Ngoc Nguyen

    (Thainguyen University of Education, Thainguyen University, Thai Nguyen, Viet Nam)

  • Phutthakine Vaciaxa

    (Khangkhay Teacher Training College, Xiangkhoang, Laos)

  • Thu Thi Mai Lo

    (Taybac University, Sonla, Viet Nam)

  • Yen Thi Hai Nguyen

    (Thainguyen University of Sciences, Thai Nguyen University, Thai Nguyen, Vietnam)

  • Nhan Thi Thanh Pham

    (Thainguyen University of Education, Thainguyen University, Thai Nguyen, Vietnam)

  • Son Van Le

    (Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam)

  • Mau Hoang Chu

    (Thai Nguyen University of Education, Thai Nguyen University, Viet Nam)

Abstract

Glycine max (L.) Merrill is a crop that brings a lot of economic and nutritive values, however soybean is quite sensitive to stress. The applications of gene technology can improve resistance of soybean plants against external stress factors. The aim of this study was to conduct a transgenic vector containing GmDREB6 gene and determine the expression of gene encoding GmDREB6 in Nicotiana tabacum before transforming into soybean plants. The GmDREB6 artificial gene was synthesized containing nucleotide fragment encoding 230 amino acids, nucleotide fragment encoding cmyc antigen and nucleotide fragments with cut-off points of XbaI/SacI enzyme pair. The results indicated that the 35S-GmDREB6-cmyc construct was designed and transferred into tobacco plants. The GmDREB6 was incorporated in the genome and was expressed in transgenic tobacco plants at the transcriptional level. The transgenic vector pBI121_GmDREB6 well worked on the model tobacco plants. Therefore, it can be used for transferring into soybean plants to enhance soybean tolerance to abiotic stress.

Suggested Citation

  • Lan Thi Ngoc Nguyen & Phutthakine Vaciaxa & Thu Thi Mai Lo & Yen Thi Hai Nguyen & Nhan Thi Thanh Pham & Son Van Le & Mau Hoang Chu, 2019. "Design of Construct Carrying GmDREB6 to Enhance Soybean Gene Expression Related to Abiotic Stress Response," European Journal of Engineering and Technology Research, European Open Science, vol. 4(6), pages 135-139, June.
    • Handle: RePEc:epw:ejeng0:v:4:y:2019:i:6:id:61384
    DOI: 10.24018/ejeng.2019.4.6.1384
    as

    Download full text from publisher

    File URL: https://eu-opensci.org/index.php/ejeng/article/view/61384
    File Function: Abstract page
    Download Restriction: no
    File URL: https://eu-opensci.org/index.php/ejeng/article/download/61384/12136
    File Function: Full text
    Download Restriction: no
    File URL: https://libkey.io/10.24018/ejeng.2019.4.6.1384?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:epw:ejeng0:v:4:y:2019:i:6:id:61384. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Support (email available below). General contact details of provider: https://eu-opensci.org/index.php/ejeng .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.