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The Combination Effect of Collagen-Chitosan Hydrogel and Injectable Platelet-Rich Fibrin Fractionation on Osteoblast Migration and Proliferation

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  • Mardha Ade Pritia

    (University Gadjah Mada, Indonesia)

  • Nurul Fatimah

    (Universitas Gadjah Mada, Indonesia)

  • Vincensia Maria Karina

    (Universitas Gadjah Mada, Indonesia)

  • Kwartarini Murdiastuti

    (Universitas Gadjah Mada, Indonesia)

Abstract

Injectable Platelet Rich Fibrin (i-PRF) is a popular periodontal regenerative therapy in dentistry. i-PRF centrifugation resulted in the fractionation of the yellow layer and buffy coat with a red layer. The red i-PRF contains a greater number of Growth Factor carrier cells than yellow layer, besides that liquid-PRF has a suitable consistency so it easy to combine with other biomaterials. Another biomaterial that can be combined with i-PRF is collagen-chitosan with hydrogel preparations. This study aims to determine the effect of a combination of collagen-chitosan hydrogel formulation with injection of platelet-rich fibrin fractionation on osteoblast cell migration and proliferation. This type of research was an experimental laboratory divided into 3 groups, which were given a combination of the collagen-chitosan hydrogel and i-PRF red layer, a combination of collagen-chitosan hydrogel and i-PRF yellow layer, and only a combination of collagen-chitosan hydrogel. Chitosan collagen hydrogel was prepared with a collagen/chitosan ratio of 25/75, then mixed with i-PRF fractionation with a ratio of 1:1. The cell proliferation test used CCK-8, while the cell migration test used scratch wound healing. Data were tested with One way ANOVA for cell migration and Two way ANOVA for cell proliferation, followed by a Post Hoc test. The results showed that migration of osteoblast cells after 24 hours in the combination group of chitosan collagen hydrogel and i-PRF red layer was higher than the other groups with significance (p

Suggested Citation

  • Mardha Ade Pritia & Nurul Fatimah & Vincensia Maria Karina & Kwartarini Murdiastuti, 2023. "The Combination Effect of Collagen-Chitosan Hydrogel and Injectable Platelet-Rich Fibrin Fractionation on Osteoblast Migration and Proliferation," European Journal of Dental and Oral Health, European Open Science, vol. 4(3), pages 22-27, April.
    • Handle: RePEc:epw:ejdent:v:4:y:2023:i:3:id:13264
    DOI: 10.24018/ejdent.2023.4.3.264
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