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Microscopic and Molecular Detection of Entamoeba histolytica in Selected Public Primary School Pupils of Bwari Area Council, F.C.T Abuja, Nigeria

Author

Listed:
  • F. R. Okusaga

    (Nasarawa State University, Nigeria)

  • R. J. Ombugadu

    (Nasarawa State University, Nigeria)

  • A. B. Yako

    (Nasarawa State University, Nigeria)

  • G. A. Amuga

    (Nasarawa State University, Nigeria)

Abstract

This study evaluated the prevalence of Entamoeba histolytica using microscopic detection and confirmed by DNA analysis using polymerase chain reaction (PCR) technique by taking samples of some public primary school pupils within Bwari Area Council of Abuja, Nigeria. Microscopic and polymerase chain reaction-restriction fragment length polymorphism analysis targeting only a small portion (228 bp) of the adh112 gene. Results showed that out of three hundred and twenty (320) stool sample screened, twenty six 26 (8.1%) were found to be positive of the parasite. The prevalence was high among pupils in school 4 with 7 (11.2%) follow by school 5 with (11.1%), school 3 with 5(8.3%), school 1 with 5(7.3%) and the lowest school 2 with (2.9%) P

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Handle: RePEc:epw:ejbio0:v:2:y:2021:i:3:id:17196
DOI: 10.24018/ejbio.2021.2.3.196
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