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Influence of Extracellular Traps (ETs) on the Differentiation of TCD4 Cell Profiles and Macrophages in Human Autologous Culture

Author

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  • Guillermina Bailo

    (National University of Córdoba, Argentina)

  • Fernando M. Rodríguez

    (National University of Córdoba, Argentina)

  • Claudia L. Carabajal-Miotti

    (National University of Córdoba, Argentina)

  • Susana G. Ruiz de Frattari

    (National University of Córdoba, Argentina)

  • Adriana H. Vargas

    (National University of Córdoba, Argentina)

  • Natalio E. González-Silva

    (National University of Córdoba, Argentina)

  • Ivon T. C. Novak

    (National University of Córdoba, Argentina)

Abstract

Background: The formation of extracellular traps (ETs) as a microbicidal functional mechanism of various leukocytes has taken on importance today and they are implicated in the pathogenesis of various diseases and have even been involved in the current pandemic. Objectives: Study ETs generated in vitro from healthy human blood leukocytes against different stimuli (LPS, fMLP) and their influence on different T cells profiles and monocyte-macrophages, in autologous cell cultures. Methods: Heparinized human blood samples were collected with ethical consent. ETs generation was performed by stimulation with LPS and fMLP. Subsequently they were isolated. ETs influence on cell profile differentiation was performed in samples without stimulation, with OVA addition samples, and OVA-ETs addition samples. This assay was observed through immunofluorescence (IF) labeling of molecules of T CD4 profile; Th17 and innate lymphoid cells 3 ILC3 by RORɣ; activation status of T cells by CD45RO; and M1 macrophage profile by iNOS. Results: Significant CD4 and CD45RO positive cells percentage were observed between paired control samples and OVA addition samples (p

Suggested Citation

  • Guillermina Bailo & Fernando M. Rodríguez & Claudia L. Carabajal-Miotti & Susana G. Ruiz de Frattari & Adriana H. Vargas & Natalio E. González-Silva & Ivon T. C. Novak, 2023. "Influence of Extracellular Traps (ETs) on the Differentiation of TCD4 Cell Profiles and Macrophages in Human Autologous Culture," European Journal of Clinical Medicine, European Open Science, vol. 4(1), pages 14-22, January.
    • Handle: RePEc:epw:clinic:v:4:y:2023:i:1:id:12240
    DOI: 10.24018/clinicmed.2023.4.1.240
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