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Human Polymorphonuclear Neutrophil Phenotypes Generated in vitro

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  • Fernando Marcos Rodríguez
  • Claudia Leonor Carabajal-Miotti
  • Susana Graciela Ruiz de Frattari
  • Adriana Haydee Vargas
  • Natalio Emilio González-Silva
  • Ivon Teresa Clara Novak

Abstract

Background: There are a variety of polymorphonuclear neutrophil phenotypes described in different species and health or disease situations. Objective: Study human neutrophil phenotypes generated in vitro. Methods: Heparinized human blood samples were collected with ethical consent. Polymorphonuclear neutrophils purification and autologous cultures was performed. Neutrophil stimulation was performed with LPS, fMLP or OVA. Immunofluorescence was applied. Results: “Polymorphonuclear neutrophil-antigen presenting cell” profile was generated in vitro, expressing CD80, CD86 and HLA-DR molecules. Immunofluorescence analysis show: CD80 expression, significant differences between CTFT control and CTFT fMLP (p

Suggested Citation

  • Fernando Marcos Rodríguez & Claudia Leonor Carabajal-Miotti & Susana Graciela Ruiz de Frattari & Adriana Haydee Vargas & Natalio Emilio González-Silva & Ivon Teresa Clara Novak, 2022. "Human Polymorphonuclear Neutrophil Phenotypes Generated in vitro," European Journal of Clinical Medicine, European Open Science, vol. 3(4), pages 21-29, July.
    • Handle: RePEc:epw:clinic:v:3:y:2022:i:4:id:12225
    DOI: 10.24018/clinicmed.2022.3.4.225
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    1. ., 2003. "Self-Organization and Economics - What is New?," Chapters, in: The Evolving Economy, chapter 10, pages 197-216, Edward Elgar Publishing.
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