Novel Two-Dimensional Copper Coordination Networks for Enzyme Inhibition: Fabrication Strategies and Mechanisms

Two-dimensional copper-based coordination polymers have emerged as promising candidates for enzyme inhibition applications, particularly in the development of urease inhibitors for agricultural and biomedical applications. This paper presents a comprehensive investigation of novel fabrication strategies for synthesizing copper coordination networks with enhanced enzyme inhibition properties. The research focuses on the strategic use of auxiliary ligands to regulate the structural properties and optimize the inhibitory performance of these materials. Through systematic design approaches, we demonstrate how V-shaped second auxiliary ligands can be employed to create highly efficient two-dimensional copper-based coordination polymers with superior urease inhibition capabilities. The fabrication methodology involves careful selection of organic linkers and metal nodes to achieve optimal pore size distribution and surface functionalization. Mechanistic studies reveal that the inhibition process occurs through competitive binding and structural disruption of the enzyme active site. The synthesized materials exhibit exceptional stability under physiological conditions and demonstrate significantly enhanced inhibition efficiency compared to conventional inhibitors. These findings provide valuable insights into the rational design of metal-organic frameworks for biomedical applications and establish a foundation for developing next-generation enzyme inhibitors with improved selectivity and efficacy.