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Effect of Bacillus subtilis on the microarchitectural development of the immune system in Salmonella-challenged broiler chickens

Author

Listed:
  • A Sikandar

    (Sub-campus, Jhang, University of Veterinary and Animal Sciences (UVAS), Lahore, Pakistan)

  • H Zaneb

    (Department of Anatomy and Histology, University of Veterinary and Animal Sciences (UVAS), Lahore, Pakistan)

  • A Nasir

    (Sub-campus, Jhang, University of Veterinary and Animal Sciences (UVAS), Lahore, Pakistan)

  • A Rehman

    (Sub-campus, Jhang, University of Veterinary and Animal Sciences (UVAS), Lahore, Pakistan)

  • M Kashif

    (Sub-campus, Jhang, University of Veterinary and Animal Sciences (UVAS), Lahore, Pakistan)

  • M Shah

    (Department of Animal Health, The University of Agriculture, Peshawar, Pakistan)

  • Z Luqman

    (Faculty of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, Bahawalpur, Pakistan)

  • S Din

    (Department of Anatomy and Histology, University of Veterinary and Animal Sciences (UVAS), Lahore, Pakistan)

  • MF Iqbal

    (Pir Mehr Ali Shah - Arid Agriculture University (PMAS AAUR), Rawalpindi, Pakistan)

  • I Khan

    (College of Veterinary Sciences and Animal Husbandry, Abdul Wali Khan University, Mardan, Pakistan)

  • I Irshad

    (Department of Pathology, University of Veterinary and Animal Sciences (UVAS), Lahore, Pakistan)

Abstract

The effect of Bacillus subtilis on the immune responses and morphometry of the immune organs was evaluated in broilers challenged with S. gallinarum. For this purpose, Salmonella-free birds (n = 240) were split into four groups with six replicates of ten birds each. Groups included an NC (negative control, non-infected + non-medicated), a PC-S (positive control, Salmonella-infected + non-medicated), an AT-S (Salmonella-infected + medicated with enrofloxacin), and a BS-S (Salmonella-infected + B. subtilis (2.0 × 1010 cfu/g; 0.1 g/kg) group. On day 21, the thickness of the thymus cortex and medulla, germinal centre area of the spleen, bursal follicular length and bursal follicular area increased (P < 0.05) in the BS-S when compared to the NC and PC-S groups. On day 35, the BS-S group exhibited a higher (P < 0.05) antibody titre against the Newcastle disease virus (NDV), and cortex of the thymus was thicker (P < 0.05) compared to the other groups. A decrease in the thymus medulla thickness, germinal area of the spleen and bursal follicular number were noted in the PC-S group when compared to the other treatment groups. In conclusion, the prophylactic use of B. subtilis type probiotics alleviated the stress resulting from a Salmonella gallinarum infection and improved the immune organs development and function in infected broilers.

Suggested Citation

  • A Sikandar & H Zaneb & A Nasir & A Rehman & M Kashif & M Shah & Z Luqman & S Din & MF Iqbal & I Khan & I Irshad, 2022. "Effect of Bacillus subtilis on the microarchitectural development of the immune system in Salmonella-challenged broiler chickens," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 67(1), pages 28-37.
    • Handle: RePEc:caa:jnlvet:v:67:y:2022:i:1:id:231-2020-vetmed
    DOI: 10.17221/231/2020-VETMED
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