IDEAS home Printed from https://ideas.repec.org/a/caa/jnlvet/v61y2016i5id8876-vetmed.html
   My bibliography  Save this article

Therapeutic effects of a combined antibiotic-enzyme treatment on subclinical mastitis in lactating dairy cows

Author

Listed:
  • B. Khoramian

    (Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran)

  • M. Emaneini

    (School of Medicine, Tehran University of Medical Sciences. Tehran, Iran)

  • M. Bolourchi

    (Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran)

  • A. Niasari-Naslaji

    (Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran)

  • A. Gorganzadeh

    (Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran)

  • S. Abani

    (Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran)

  • P. Hovareshti

    (Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran)

Abstract

ABSTRACT: The objective of this study was to evaluate a combined antibiotic-enzyme therapy for Staphylococcus aureus mastitis and biofilm formation. A total of 141 cases of S. aureus chronic mastitis from three farms were divided into two groups: the control group (n = 54) were treated with Nafpenzal® ointment; the enzyme + antibiotic group (n = 87) were treated with Nafpenzal® plus an enzymatic ointment (MastiVeyxym®). Quantitative determination of biofilm formation was determined using a colorimetric microplate assay and the detection of ica genes by PCR. Enzyme + antibiotic therapy did not significantly improve cure rates compared to control (48.3 vs 38.9%). The cure rate for infections caused by biofilm-positive strains was 42.6 and 46.6% for control and enzyme + antibiotic groups, respectively (P > 0.05). Comparison of cure rates between farms showed a relationship with somatic cell count (SCC), parity and oxacillin resistance. 79.4% of the isolates produced biofilm and antibacterial resistance rates for oxacillin, penicillin and streptomycin were 25.5, 71.6 and 95.7%, respectively. These results indicate that while the increase in mastitis cure rate using enzymatic therapy was not significant, this treatment could be useful in some situations.

Suggested Citation

  • B. Khoramian & M. Emaneini & M. Bolourchi & A. Niasari-Naslaji & A. Gorganzadeh & S. Abani & P. Hovareshti, 2016. "Therapeutic effects of a combined antibiotic-enzyme treatment on subclinical mastitis in lactating dairy cows," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 61(5), pages 237-242.
    • Handle: RePEc:caa:jnlvet:v:61:y:2016:i:5:id:8876-vetmed
    DOI: 10.17221/8876-VETMED
    as

    Download full text from publisher

    File URL: http://vetmed.agriculturejournals.cz/doi/10.17221/8876-VETMED.html
    Download Restriction: free of charge
    File URL: http://vetmed.agriculturejournals.cz/doi/10.17221/8876-VETMED.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/8876-VETMED?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlvet:v:61:y:2016:i:5:id:8876-vetmed. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.