IDEAS home Printed from https://ideas.repec.org/a/caa/jnlvet/v60y2015i9id8437-vetmed.html
   My bibliography  Save this article

Relationship between acrosome integrity changes and in vitro fertilising ability of bovine spermatozoa

Author

Listed:
  • Z. Reckova

    (Mendel University, Brno, Czech Republic)

  • M. Machatkova

    (Veterinary Research Institute, Brno, Czech Republic)

  • L. Machal

    (Mendel University, Brno, Czech Republic)

  • M. Jeseta

    (Veterinary Research Institute, Brno, Czech Republic)

Abstract

This study was designed to investigate the characteristics of acrosomal changes during capacitation of bovine spermatozoa in relationship to in vitro fertility of individual bulls. Motile spermatozoa were separated from frozen-thawed semen by a swim-up procedure and capacitated in IVF-TALP medium with or without heparin. The spermatozoa were evaluated in terms of acrosomal changes at 0, 3, 4, 5, 6 and 8 h of capacitation. Proportions of acrosome-reacted spermatozoa at 5 h and 0 h of capacitation were used for calculation of the heparin response index. Variations in the heparin response index were found among individual bulls. Based on the mean response index value of all bulls, they fell into three categories: bulls with greater, intermediate and no response to heparin (GRH, IRH and NRH, respectively). Differences in the heparin response index between the bull categories were significant (P < 0.05). Higher D7 and D8 embryo development rates were found in the IRH vs. NRH bulls (P < 0.05). In conclusion, this study shows that the spermatozoa of bulls with a greater or intermediate response to heparin appear to be most suitable for in vitro embryo production compared with spermatozoa of bulls with no response to heparin.

Suggested Citation

  • Z. Reckova & M. Machatkova & L. Machal & M. Jeseta, 2015. "Relationship between acrosome integrity changes and in vitro fertilising ability of bovine spermatozoa," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 60(9), pages 469-475.
    • Handle: RePEc:caa:jnlvet:v:60:y:2015:i:9:id:8437-vetmed
    DOI: 10.17221/8437-VETMED
    as

    Download full text from publisher

    File URL: http://vetmed.agriculturejournals.cz/doi/10.17221/8437-VETMED.html
    Download Restriction: free of charge
    File URL: http://vetmed.agriculturejournals.cz/doi/10.17221/8437-VETMED.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/8437-VETMED?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    References listed on IDEAS

    as
    1. M. Machatkova & K. Hanzalova & J. Horakova & Z. Reckova & P. Hulinska, 2006. "Collection of oocytes from donors in the growth phase of follicular development can enhance the production of bovine embryos for cryopreservation," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 51(5), pages 232-238.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. M. Machatkova & P. Hulinska & Z. Reckova & K. Hanzalova & J. Spanihelova & R. Pospisil, 2008. "In vitro production of embryos from high performance cows and the development of frozen-thawed embryos after transfer: a field study," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 53(7), pages 358-364.

    More about this item

    Keywords

    bull; spermatozoa; acrosome; embryos; IVF;
    All these keywords.

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlvet:v:60:y:2015:i:9:id:8437-vetmed. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.