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Identification of heterosomes in spermatoza of rams with 54,XX/54,XY chimerism

Author

Listed:
  • A. Kozubska-Sobocinska

    (National Research Institute of Animal Production, Balice n. Krakow, Poland)

  • B. Rejduch

    (National Research Institute of Animal Production, Balice n. Krakow, Poland
    Department of Biotechnology, University of Rzeszow, Kolbuszowa n. Rzeszow, Poland)

Abstract

The aim of the study was to identify heterosomes in the semen of three Romanov rams - carriers of leukocyte chimerism (FISH technique) and to determine the proportions between spermatozoa with X and Y chromosomes. The choice of bovine probes for hybridization with ram heterosomes was dictated by genetic conservatism of bovine and ovine heterosomes. The ratio between spermatozoa with a yellow fluorescent signal containing the X chromosome in the haploid set and spermatozoa with a red-purple signal indicating the presence of the Y chromosome, taking into account spermatozoa with no signal, was 52%:43%:5% in ram No. PL100006077676; 47%:44%:9% in ram No. PL100006078031; and 48%:46%:6% in ram No. PL100006078895. The results obtained lead us to conclude that the 54,XX/54,XY chimerism has no effect on sex ratio in offspring.

Suggested Citation

  • A. Kozubska-Sobocinska & B. Rejduch, 2008. "Identification of heterosomes in spermatoza of rams with 54,XX/54,XY chimerism," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 53(5), pages 250-254.
  • Handle: RePEc:caa:jnlvet:v:53:y:2008:i:5:id:1855-vetmed
    DOI: 10.17221/1855-VETMED
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